Utility of the Interferon-Gamma Enzyme-Linked Immunosorbent Spot Assay to Predict Risk of Cytomegalovirus Infection in Kidney Transplant Recipients
Issued Date
2023-01-01
Resource Type
ISSN
09340874
eISSN
14322277
Scopus ID
2-s2.0-85182689802
Pubmed ID
38249787
Journal Title
Transplant International
Volume
36
Rights Holder(s)
SCOPUS
Bibliographic Citation
Transplant International Vol.36 (2023)
Suggested Citation
Namsiripongpun W., Kantachuvesiri S., Bruminhent J. Utility of the Interferon-Gamma Enzyme-Linked Immunosorbent Spot Assay to Predict Risk of Cytomegalovirus Infection in Kidney Transplant Recipients. Transplant International Vol.36 (2023). doi:10.3389/ti.2023.11527 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/95902
Title
Utility of the Interferon-Gamma Enzyme-Linked Immunosorbent Spot Assay to Predict Risk of Cytomegalovirus Infection in Kidney Transplant Recipients
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Abstract
Non‐specific interferon‐gamma (IFN‐γ) enzyme‐linked immunosorbent (ELISpot) responses after solid organ transplant (SOT) and their relationship with cytomegalovirus (CMV) reactivation have hardly been investigated. Adult kidney transplant (KT) recipients underwent measurement of IFN‐γ‐producing T cells using the ELISpot assay before and 1 month after transplantation. Data for CMV infection episodes were collected. Risk factors for post‐transplant CMV infection, based on IFN‐γ responses, were analyzed using a Cox proportional hazards model. A total of 93 KT recipients were enrolled in the study and 84 evaluable participants remained at 1 month post KT. Thirty-three (39%) recipients developed subsequent CMV infection within 6 months post‐transplant. At 1‐month post‐transplant, IFN‐γ‐producing T cells with <250 spot‐forming units (SFUs)/2.5 × 105 peripheral blood mononuclear cells (PBMCs) were significantly associated with CMV infection (HR 3.1, 95% CI 1.4–7.1, p = 0.007). On multivariable analysis, posttransplant IFN‐γ‐producing T cells with <250 SFUs/2.5 × 105 PBMCs remained independently associated with CMV infection (HR 3.1, 95% CI 1.2–7.8, p = 0.019). Conclusions: KT recipients with low IFN‐γ‐producing T cells measured by the ELISpot assay are more likely to develop CMV infection after transplantation. Therefore, measurement of nonspecific cell-mediated immunity ELISpot responses could potentially stratify recipients at risk of CMV infection (Thai Clinical Trials Registry, TCTR20210216004).