Label-Free Evaluation of Maturation and Hepatotoxicity of Human iPSC-Derived Hepatocytes Using Hyperspectral Raman Imaging
Issued Date
2023-01-01
Resource Type
ISSN
00032700
eISSN
15206882
Scopus ID
2-s2.0-85163717790
Journal Title
Analytical Chemistry
Rights Holder(s)
SCOPUS
Bibliographic Citation
Analytical Chemistry (2023)
Suggested Citation
Li M., Ueyama-Toba Y., Lindley M., Kongklad G., Nawa Y., Kumamoto Y., Ishida S., Kanda Y., Fujita S., Mizuguchi H., Fujita K. Label-Free Evaluation of Maturation and Hepatotoxicity of Human iPSC-Derived Hepatocytes Using Hyperspectral Raman Imaging. Analytical Chemistry (2023). doi:10.1021/acs.analchem.3c00976 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87988
Title
Label-Free Evaluation of Maturation and Hepatotoxicity of Human iPSC-Derived Hepatocytes Using Hyperspectral Raman Imaging
Other Contributor(s)
Abstract
To promote the clinical application of human induced pluripotent stem cell (hiPSC)-derived hepatocytes, a method capable of monitoring regenerative processes and assessing differentiation efficiency without harming or modifying these cells is important. Raman microscopy provides a powerful tool for this as it enables label-free identification of intracellular biomolecules in live samples. Here, we used label-free Raman microscopy to assess hiPSC differentiation into hepatocyte lineage based on the intracellular chemical content. We contrasted these data with similar phenotypes from the HepaRG and from commercially available hiPSC-derived hepatocytes (iCell hepatocytes). We detected hepatic cytochromes, lipids, and glycogen in hiPSC-derived hepatocyte-like cells (HLCs) but not biliary-like cells (BLCs), indicating intrinsic differences in biomolecular content between these phenotypes. The data show significant glycogen and lipid accumulation as early as the definitive endoderm transition. Additionally, we explored the use of Raman imaging as a hepatotoxicity assay for the HepaRG and iCell hepatocytes, with data displaying a dose-dependent reduction of glycogen accumulation in response to acetaminophen. These findings show that the nondestructive and high-content nature of Raman imaging provides a promising tool for both quality control of hiPSC-derived hepatocytes and hepatotoxicity screening.