Tracking Plasmodium knowlesi Through Fecal DNA for Monitoring Zoonotic Transmission in Wild Macaques Across Southeast and South Asia
Issued Date
2026-05-15
Resource Type
ISSN
00221899
eISSN
15376613
Scopus ID
2-s2.0-105039178882
Pubmed ID
41766625
Journal Title
Journal of Infectious Diseases
Volume
233
Issue
5
Start Page
e1238
End Page
e1243
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Infectious Diseases Vol.233 No.5 (2026) , e1238-e1243
Suggested Citation
Wannigama D.L., Amarasiri M., Phattharapornjaroen P., Hurst C., Modchang C., Cynthia B., Miyanaga K., Cui L., Fernandez S., Melhem N.M., Luk-in S., Singer A.C., Ragupathi N.K.D., Htun T.S., Sei K., Ngamwongsatit N., Hui N.S., Shimotai Y., Ounjai P., Kanthawee P., Tacharoenmuang R., Kauba A., Eang C., Romone L., Dharne M., de Araujo J.C., Ndatuwong L.G., Werawatte W.K.C.P., Chanthasiri T., Zhao J., Mori H., Besa J.J.V., Kurt Ö., Kanjanabuch T., Zahraei-Ramazani A.R., Higgins P.G., Aoyagi T., Kicic A., Trowsdale S., Hongsing P., Yang X., Wang Y., Khatib A., Sano D., Shibuya K., Abe S., Hamamoto H. Tracking Plasmodium knowlesi Through Fecal DNA for Monitoring Zoonotic Transmission in Wild Macaques Across Southeast and South Asia. Journal of Infectious Diseases Vol.233 No.5 (2026) , e1238-e1243. e1243. doi:10.1093/infdis/jiag128 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116944
Title
Tracking Plasmodium knowlesi Through Fecal DNA for Monitoring Zoonotic Transmission in Wild Macaques Across Southeast and South Asia
Author(s)
Wannigama D.L.
Amarasiri M.
Phattharapornjaroen P.
Hurst C.
Modchang C.
Cynthia B.
Miyanaga K.
Cui L.
Fernandez S.
Melhem N.M.
Luk-in S.
Singer A.C.
Ragupathi N.K.D.
Htun T.S.
Sei K.
Ngamwongsatit N.
Hui N.S.
Shimotai Y.
Ounjai P.
Kanthawee P.
Tacharoenmuang R.
Kauba A.
Eang C.
Romone L.
Dharne M.
de Araujo J.C.
Ndatuwong L.G.
Werawatte W.K.C.P.
Chanthasiri T.
Zhao J.
Mori H.
Besa J.J.V.
Kurt Ö.
Kanjanabuch T.
Zahraei-Ramazani A.R.
Higgins P.G.
Aoyagi T.
Kicic A.
Trowsdale S.
Hongsing P.
Yang X.
Wang Y.
Khatib A.
Sano D.
Shibuya K.
Abe S.
Hamamoto H.
Amarasiri M.
Phattharapornjaroen P.
Hurst C.
Modchang C.
Cynthia B.
Miyanaga K.
Cui L.
Fernandez S.
Melhem N.M.
Luk-in S.
Singer A.C.
Ragupathi N.K.D.
Htun T.S.
Sei K.
Ngamwongsatit N.
Hui N.S.
Shimotai Y.
Ounjai P.
Kanthawee P.
Tacharoenmuang R.
Kauba A.
Eang C.
Romone L.
Dharne M.
de Araujo J.C.
Ndatuwong L.G.
Werawatte W.K.C.P.
Chanthasiri T.
Zhao J.
Mori H.
Besa J.J.V.
Kurt Ö.
Kanjanabuch T.
Zahraei-Ramazani A.R.
Higgins P.G.
Aoyagi T.
Kicic A.
Trowsdale S.
Hongsing P.
Yang X.
Wang Y.
Khatib A.
Sano D.
Shibuya K.
Abe S.
Hamamoto H.
Author's Affiliation
The University of Queensland
Monash University
Tohoku University
The University of Sheffield
University of Toronto Faculty of Medicine
University of Auckland
Universidade Federal de Minas Gerais
Curtin University
Mahidol University
Chulalongkorn University
Academy of Scientific and Innovative Research (AcSIR)
American University of Beirut
Jichi Medical University
Graduate School of Medicine
Thammasat University
UK Centre for Ecology & Hydrology
National Chemical Laboratory India
Juntendo University School of Medicine
Faculty of Science, Mahidol University
Yamagata University Faculty of Medicine
The Kids Research Institute Australia
Medizinische Fakultät
Acıbadem Mehmet Ali Aydınlar Üniversitesi
School of Public Health
King Chulalongkorn Memorial Hospital
Faculty of Medicine, Chulalongkorn University
UWA Medical School
Mae Fah Luang University
Ministry of Higher Education, Science, Research and Innovation
Perth Children's Hospital
Faculty of Medicine, Thammasat University
University of Malaya Medical Centre
University of the Philippines College of Medicine
Armed Forces Research Institute of Medical Sciences, Thailand
Chulabhorn Royal Academy
Wayamba University of Sri Lanka
Yamagata Prefectural Central Hospital
Kitasato University Graduate School of Medical Science
Royal University of Phnom Penh
The Tokyo Foundation for Policy Research
Yamagata Prefectural University of Health Sciences
Hospital of Chongqing University
Adamawa State University, Mubi
Mahidol University, Center of Excellence for Vectors and Vector-Borne Diseases
St. Carolus Hospital
University of Goroka
Centre of Excellence in Mathematics
Partner Site Bonn-Cologne
National Institute of Health
Fuyang Women and Children's Hospital
Bioberrys Healthcare and Research Centre
Vanuatu National Museum
Mahosot Hospital
So Pyay Clinic
Monash University
Tohoku University
The University of Sheffield
University of Toronto Faculty of Medicine
University of Auckland
Universidade Federal de Minas Gerais
Curtin University
Mahidol University
Chulalongkorn University
Academy of Scientific and Innovative Research (AcSIR)
American University of Beirut
Jichi Medical University
Graduate School of Medicine
Thammasat University
UK Centre for Ecology & Hydrology
National Chemical Laboratory India
Juntendo University School of Medicine
Faculty of Science, Mahidol University
Yamagata University Faculty of Medicine
The Kids Research Institute Australia
Medizinische Fakultät
Acıbadem Mehmet Ali Aydınlar Üniversitesi
School of Public Health
King Chulalongkorn Memorial Hospital
Faculty of Medicine, Chulalongkorn University
UWA Medical School
Mae Fah Luang University
Ministry of Higher Education, Science, Research and Innovation
Perth Children's Hospital
Faculty of Medicine, Thammasat University
University of Malaya Medical Centre
University of the Philippines College of Medicine
Armed Forces Research Institute of Medical Sciences, Thailand
Chulabhorn Royal Academy
Wayamba University of Sri Lanka
Yamagata Prefectural Central Hospital
Kitasato University Graduate School of Medical Science
Royal University of Phnom Penh
The Tokyo Foundation for Policy Research
Yamagata Prefectural University of Health Sciences
Hospital of Chongqing University
Adamawa State University, Mubi
Mahidol University, Center of Excellence for Vectors and Vector-Borne Diseases
St. Carolus Hospital
University of Goroka
Centre of Excellence in Mathematics
Partner Site Bonn-Cologne
National Institute of Health
Fuyang Women and Children's Hospital
Bioberrys Healthcare and Research Centre
Vanuatu National Museum
Mahosot Hospital
So Pyay Clinic
Corresponding Author(s)
Other Contributor(s)
Abstract
We conducted the noninvasive surveillance of Plasmodium knowlesi in wild macaques using 4752 fecal samples collected across 9 endemic countries. Parasite DNA was detected in 390 samples (8.2%), with positivity rates ranging from 1.4% to 18.4%. This provides the first field-based evidence that P. knowlesi DNA in feces shed by macaques and present under natural conditions can be detected. These findings validate fecal sampling as a practical and scalable tool for tracking zoonotic-malaria. The results support integration into forest-runoff and rural wastewater surveillance systems, offering new opportunities for early detection of pathogens and environmental monitoring at the human–wildlife interface.