The effects of short neuropeptide F on ovarian maturation and spawning in female giant freshwater prawn, Macrobrachium rosenbergii, and associated regulatory mechanisms
Issued Date
2023-05-15
Resource Type
ISSN
00448486
Scopus ID
2-s2.0-85148735484
Journal Title
Aquaculture
Volume
569
Rights Holder(s)
SCOPUS
Bibliographic Citation
Aquaculture Vol.569 (2023)
Suggested Citation
Tinikul Y., Tinikul R., Engsusophon A., Sobhon P. The effects of short neuropeptide F on ovarian maturation and spawning in female giant freshwater prawn, Macrobrachium rosenbergii, and associated regulatory mechanisms. Aquaculture Vol.569 (2023). doi:10.1016/j.aquaculture.2023.739361 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81426
Title
The effects of short neuropeptide F on ovarian maturation and spawning in female giant freshwater prawn, Macrobrachium rosenbergii, and associated regulatory mechanisms
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
In the present study, we investigated the effects of two short neuropeptide F (Mro-sNPF) mature sequences, namely APALRLRFamide and DRTPALRLRFamide, on ovarian maturation and spawning in Macrobrachium rosenbergii females. At doses of 10−5 and 10−6 mol/prawn, both Mro-sNPF peptides significantly shortened the ovarian developmental period (P < 0.05), but enhanced the gonadosomatic index (GSI), oocyte diameter (OD), and cell proliferation, compared with control values (P < 0.05). However, at the lowest dose (10−7 mol/prawn), neither peptide significantly affected ovarian maturation, GSI, nor OD compared with the control values. Females treated with the two peptides at the dose of 10−5 or 10−6 mol/prawn attained mature oocyte stages faster than did control females. Treatment with both peptides significantly elevated hemolymph vitellogenin concentrations and upregulated MrEcR transcripts, compared with the control treatment (P < 0.05). Finally, both Mro-sNPFs induced spawning by shortening its duration by half (∼1.5 days) compared with the control duration (∼3.1 days) (P < 0.05). The number of eggs per spawn was approximately 15.4 × 104 eggs in the experimental group and in the control group (∼15.35 × 104 eggs), albeit without significance. Therefore, among the treatment groups, using APALRLRFamide at 10−5 mol/prawn showed promising effects on the induction of ovarian maturation and spawning. Taken together, Mro-sNPF peptides can promote ovarian development and spawning, indicating their potential application to stimulate gamete and larval production in this important crustacean aquaculture species.