Inhibition of the norA gene expression and the NorA efflux pump by the tannic acid
Issued Date
2023-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85174180910
Journal Title
Scientific Reports
Volume
13
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.13 No.1 (2023)
Suggested Citation
Tintino S.R., Wilairatana P., de Souza V.C.A., da Silva J.M.A., Pereira P.S., de Morais Oliveira-Tintino C.D., de Matos Y.M.L.S., Júnior J.T.C., de Queiroz Balbino V., Siqueira-Junior J.P., Menezes I.R.A., Siyadatpanah A., Coutinho H.D.M., Balbino T.C.L. Inhibition of the norA gene expression and the NorA efflux pump by the tannic acid. Scientific Reports Vol.13 No.1 (2023). doi:10.1038/s41598-023-43038-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/90725
Title
Inhibition of the norA gene expression and the NorA efflux pump by the tannic acid
Other Contributor(s)
Abstract
The NorA efflux pump of Staphylococcus aureus is known to play a major role in the development of resistance against quinolone drugs by reducing their concentration inside target pathogens. The objective of this study was to evaluate the ability of tannic acid to inhibit the gene expression of the NorA efflux pump in Staphylococcus aureus and to evaluate the in silico effect on the pump. Efflux pump inhibition was evaluated by fluorimetry. The checkerboard method evaluates the effect of the test substance in combination with an antimicrobial at different concentrations. To gene expression evaluation NorA the assay was performed using: a sub-inhibitory concentration preparation (MIC/4) of the antibiotic; a sub-inhibitory concentration preparation (MIC/4) of the antibiotic associated with tannic acid at a sub-inhibitory concentration (MIC/4). In this study, docking simulations were performed by the SWISSDOCK webserver. The ability of tannic acid to inhibit the NorA efflux pump can be related to both the ability to inhibit the gene expression of this protein, acting on signaling pathways involving the ArlRS membrane sensor. As well as acting directly through direct interaction with the NorA protein, as seen in the approach and in silico and in vitro per checkerboard method and fluorimetry of bromide accumulated in the cell.