A prospective method for non-invasive diagnosis of iron deficiency anemia using microfluidic paper-based analytical devices and a natural reagent from red rose (Rosa damascena) flower extract
Issued Date
2025-09-11
Resource Type
ISSN
17599660
eISSN
17599679
Scopus ID
2-s2.0-105015407458
Pubmed ID
40839348
Journal Title
Analytical Methods
Volume
17
Issue
35
Start Page
6989
End Page
6997
Rights Holder(s)
SCOPUS
Bibliographic Citation
Analytical Methods Vol.17 No.35 (2025) , 6989-6997
Suggested Citation
Sulistyarti H., Izzah U.N., Efrieza N.J., Valentina E., Susianti H., Mulyasuryani A., Sabarudin A., Nacapricha D., Utama M.M. A prospective method for non-invasive diagnosis of iron deficiency anemia using microfluidic paper-based analytical devices and a natural reagent from red rose (Rosa damascena) flower extract. Analytical Methods Vol.17 No.35 (2025) , 6989-6997. 6997. doi:10.1039/d5ay00928f Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/112131
Title
A prospective method for non-invasive diagnosis of iron deficiency anemia using microfluidic paper-based analytical devices and a natural reagent from red rose (Rosa damascena) flower extract
Author's Affiliation
Corresponding Author(s)
Other Contributor(s)
Abstract
A non-invasive, low-cost, non-toxic, and user-friendly microfluidic paper-based analytical device (μPAD) was developed for Fe(ii) determination in artificial urine samples. The determination method is based on the color change of anthocyanin from Rosa damascena flower extract (RDFE) through the formation of an Fe(ii)-anthocyanin complex. The image of the complex was captured using a smartphone camera and analyzed for color intensity using ImageJ. The “mickey mouse” hydrophobic pattern on the μPAD was created by printing a pattern consisting of one circular sample zone, two circular detection zones of the same size (7.5 mm i.d.), and two channel zones with dimensions of 3.3 × 2.5 mm. The optimal conditions for the μPAD were 1.2 μL of extract (8% v/v), 28 μL of the sample, and a reaction time of 25 minutes. The proposed μPAD method offers a linear dynamic range of 0-4 mg L<sup>−1</sup>, with LOD and LOQ values of 0.075 and 0.25 mg L<sup>−1</sup>, respectively. The validation of the method using recovery tests yielded results ranging from 93% to 106%, which are considered accurate. This method successfully determined Fe(ii) ions in artificial urine samples, with Fe(ii) levels mimicking those of patients with iron deficiency anemia, and it is highly applicable to other sample types.