LL-37 and bisphosphonate co-delivery 3D-scaffold with antimicrobial and antiresorptive activities for bone regeneration
Issued Date
2024-10-01
Resource Type
ISSN
01418130
eISSN
18790003
Scopus ID
2-s2.0-85200011698
Pubmed ID
39059543
Journal Title
International Journal of Biological Macromolecules
Volume
277
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Biological Macromolecules Vol.277 (2024)
Suggested Citation
Ye P., Yang Y., Qu Y., Yang W., Tan J., Zhang C., Sun D., Zhang J., Zhao W., Guo S., Song L., Hou T., Zhang Z., Tang Y., Limjunyawong N., Xu J., Dong S., Dou C., Luo F. LL-37 and bisphosphonate co-delivery 3D-scaffold with antimicrobial and antiresorptive activities for bone regeneration. International Journal of Biological Macromolecules Vol.277 (2024). doi:10.1016/j.ijbiomac.2024.134091 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/100257
Title
LL-37 and bisphosphonate co-delivery 3D-scaffold with antimicrobial and antiresorptive activities for bone regeneration
Corresponding Author(s)
Other Contributor(s)
Abstract
This study introduces a novel 3D scaffold for bone regeneration, composed of silk fibroin, chitosan, nano-hydroxyapatite, LL-37 antimicrobial peptide, and pamidronate. The scaffold addresses a critical need in bone tissue engineering by simultaneously combating bone infections and promoting bone growth. LL-37 was incorporated for its broad-spectrum antimicrobial properties, while pamidronate was included to inhibit bone resorption. The scaffold's porous structure, essential for cell infiltration and nutrient diffusion, was achieved through a freeze-drying process. In vitro assessments using SEM and FTIR confirmed the scaffold's morphology and chemical integrity. Antimicrobial efficacy was tested against pathogens of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). In vivo studies in a murine model of infectious bone defect revealed the scaffold's effectiveness in reducing inflammation and bacterial load, and promoting bone regeneration. RNA sequencing of treated specimens provided insights into the molecular mechanisms underlying these observations, revealing significant gene expression changes related to bone healing and immune response modulation. The results indicate that the scaffold effectively inhibits bacterial growth and supports bone cell functions, making it a promising candidate for treating infectious bone defects. Future studies should focus on optimizing the release of therapeutic agents and evaluating the scaffold's clinical potential.
