In vitro shoot organogenesis in Anthurium andraeanum 'HC 028' - a highly valued cut-flower and potted ornamental plant

Abstract

A high-frequency clonal propagation protocol was developed for Anthurium andraeanum 'HC 028', a highly valued a cut-flower and potted ornamental plant. Shoot tip explants of Anthurium andraeanum 'HC 028' were cultured on MS medium fortified with different concentrations of BAP (1.0-5.0 mg L-1) and kinetin (1.0-5.0 mg L-1) individually for shoot induction. The highest frequency (100%) of multiple shoot regeneration with maximum number of shoots (12.90 shoots explant-1) was noticed on MS medium supplemented with 4.0 mg L-1 BAP after being culture for 60 days. Elongated shoots dissected out from the in vitro proliferated shoot clumps were cultured on MS medium fortified with various concentrations (1.0-3.0 mg L-1) of auxins (NAA and IBA) for root induction. Highest frequency of rooting (100%) was noticed on MS medium augmented with 2.0 mg L-1 IBA. The plantlets were successfully acclimatized in the greenhouse with more than 95% survival rate. Regenerated plantlets were morphologically identical with mother plants. The present in vitro propagation protocol would facilitate an alternative method for rapid and large-scale production of Anthurium andraeanum 'HC 028' a cut-flower and potted ornamental plant.