Distinctive Roles of YAP and TAZ in Human Endothelial Progenitor Cells Growth and Functions
Issued Date
2022-01-01
Resource Type
eISSN
22279059
Scopus ID
2-s2.0-85123582984
Journal Title
Biomedicines
Volume
10
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biomedicines Vol.10 No.1 (2022)
Suggested Citation
Klaihmon P., Lorthongpanich C., Kheolamai P., Luanpitpong S., Issaragrisil S. Distinctive Roles of YAP and TAZ in Human Endothelial Progenitor Cells Growth and Functions. Biomedicines Vol.10 No.1 (2022). doi:10.3390/biomedicines10010147 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83929
Title
Distinctive Roles of YAP and TAZ in Human Endothelial Progenitor Cells Growth and Functions
Author's Affiliation
Other Contributor(s)
Abstract
The hippo signaling pathway plays an essential role in controlling organ size and balancing tissue homeostasis. Its two main effectors, yes-associated protein (YAP) and WW domain-containing transcription regulator 1, WWTR1 or TAZ, have also been shown to regulate endothelial cell functions and angiogenesis. In this study, the functions of YAP and TAZ in human endothelial progenitor cells (EPCs) were investigated by a loss-of-function study using CRISPR/Cas9-mediated gene knockdown (KD). Depletion of either YAP or TAZ reduced EPC survival and impaired many of their critical functions, including migration, invasion, vessel-formation, and expression of pro-angiogenic genes. Notably, TAZ-KD EPCs exhibited more severe phenotypes in comparison to YAP-KD EPCs. Moreover, the conditioned medium derived from TAZ-KD EPCs reduced the survivability of human lung cancer cells and increased their sensitivity to chemotherapeutic agents. The overexpression of either wild-type or constitutively active TAZ rescued the impaired phenotypes of TAZ-KD EPCs and restored the expression of pro-angiogenic genes in those EPCs. In summary, we demonstrate the crucial role of Hippo signaling components, YAP and TAZ, in controlling several aspects of EPC functions that can potentially be used as a drug target to enhance EPC functions in patients.
