Nanocoating and biological evaluation of clindamycin- and rifampicin-loaded nanospheres impregnated silicone tube for antibacterial application
Issued Date
2022-01-01
Resource Type
ISSN
10837450
eISSN
10979867
Scopus ID
2-s2.0-85130072312
Pubmed ID
35400277
Journal Title
Pharmaceutical Development and Technology
Volume
27
Issue
3
Start Page
372
End Page
378
Rights Holder(s)
SCOPUS
Bibliographic Citation
Pharmaceutical Development and Technology Vol.27 No.3 (2022) , 372-378
Suggested Citation
Thanongsak W., Boongird A., Nasongkla N. Nanocoating and biological evaluation of clindamycin- and rifampicin-loaded nanospheres impregnated silicone tube for antibacterial application. Pharmaceutical Development and Technology Vol.27 No.3 (2022) , 372-378. 378. doi:10.1080/10837450.2022.2063890 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86897
Title
Nanocoating and biological evaluation of clindamycin- and rifampicin-loaded nanospheres impregnated silicone tube for antibacterial application
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Two types of nanospheres, namely clindamycin-loaded nanospheres (CN) and rifampicin-loaded nanospheres (RN) were successfully prepared using emulsion solvent evaporation technique. Poly(ε-caprolactone-random-d,l-lactide)-block-poly(ethylene glycol)-block-poly(ε-caprolactone-random-d,l-lactide) (PLEC) was used for preparing nanospheres. CN and RN were coated on an antibiotic-impregnated silicone tube surface by spray coating technique. The formulations of antibiotic-loaded nanospheres (CN and RN) and the amount of spray coating cycles were optimized. Drug-loading content (DLC), encapsulation efficiency, zeta potential, and size of nanospheres were characterized. The optimal ratios of PLEC to clindamycin; and PLEC to rifampicin were both at 2:1. Drug-loading contents of CN and RN were 2.62 ± 0.04% and 7.02 ± 0.02%, respectively. The optimum coating of 90 cycles was provided DLCs of 0.0637 ± 0.033 wt.% and 0.0613 ± 0.018 wt.% for rifampicin and clindamycin, respectively. The coating of silicone tube was able to prolong the release of both antibiotics for at least 30 days. Antibacterial activity test was performed using MRSA (ATCC 43300) and S. Epidermidis (ATCC 12228). Moreover, the cytotoxicity of antibiotic-loaded nanospheres coated on silicone tubes was evaluated. The coated silicone tube showed antibacterial activity for at least 28 days and was biocompatible with L929 cells.