Metabarcoding study to reveal the structural community of strongylid nematodes in domesticated horses in Thailand
Issued Date
2024-12-01
Resource Type
eISSN
17466148
Scopus ID
2-s2.0-85185934288
Pubmed ID
38395874
Journal Title
BMC Veterinary Research
Volume
20
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
BMC Veterinary Research Vol.20 No.1 (2024)
Suggested Citation
Hamad M.H., Islam S.I., Jitsamai W., Chinkangsadarn T., Naraporn D., Ouisuwan S., Taweethavonsawat P. Metabarcoding study to reveal the structural community of strongylid nematodes in domesticated horses in Thailand. BMC Veterinary Research Vol.20 No.1 (2024). doi:10.1186/s12917-024-03934-y Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97453
Title
Metabarcoding study to reveal the structural community of strongylid nematodes in domesticated horses in Thailand
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Abstract
Background: Mixed strongylid infections significantly impact equine health and performance. Traditional microscopy-based methods exhibit limitations in accurately identifying strongylid species. Nemabiome deep amplicon sequencing approach previously succeeded in describing the strongylid communities in livestock including equids. However, there are no available studies that describe the structural communities of strongylid parasites in horses in Thailand. Therefore, this study was undertaken encompassing the ITS-2 rDNA metabarcoding assay to characterize strongylid species within horse fecal samples collected from a cohort of yearlings at the largest domesticated stud farm in Thailand. In addition, to investigate the capability of ITS-2 rDNA in assessing the phylogenetic relationships among the identified strongylid species. Results: The study identified 14 strongylid species in the examined equine populations, each with varying prevalence. Notably, Cylicocyclus nassatus and Cylicostephanus longibursatus were identified as the predominant species, with Strongylus spp. conspicuously absent. The phylogenetic analysis of 207 amplicon sequence variants (ASVs) displayed a complex relationship among the investigated cyathostomin species, with some species are positioned across multiple clades, demonstrating close associations with various species and genera. Conclusion: The ITS-2 nemabiome sequencing technique provided a detailed picture of horse strongylid parasite species in the studied population. This establishes a foundation for future investigations into the resistance status of these parasites and enables efforts to mitigate their impact.