Target Enrichment Metagenomics Reveals Human Pegivirus-1 in Pediatric Hematopoietic Stem Cell Transplantation Recipients
Issued Date
2022-04-01
Resource Type
eISSN
19994915
Scopus ID
2-s2.0-85128801469
Pubmed ID
35458526
Journal Title
Viruses
Volume
14
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
Viruses Vol.14 No.4 (2022)
Suggested Citation
Ludowyke N., Phumiphanjarphak W., Apiwattanakul N., Manopwisedjaroen S., Pakakasama S., Sensorn I., Pasomsub E., Chantratita W., Hongeng S., Aiewsakun P., Thitithanyanont A. Target Enrichment Metagenomics Reveals Human Pegivirus-1 in Pediatric Hematopoietic Stem Cell Transplantation Recipients. Viruses Vol.14 No.4 (2022). doi:10.3390/v14040796 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84998
Title
Target Enrichment Metagenomics Reveals Human Pegivirus-1 in Pediatric Hematopoietic Stem Cell Transplantation Recipients
Author's Affiliation
Other Contributor(s)
Abstract
Human pegivirus-1 (HPgV-1) is a lymphotropic human virus, typically considered nonpathogenic, but its infection can sometimes cause persistent viremia both in immunocompetent and immunosuppressed individuals. In a viral discovery research program in hematopoietic stem cell transplant (HSCT) pediatric patients, HPgV-1 was detected in 3 out of 14 patients (21.4%) using a target enrichment next-generation sequencing method, and the presence of the viruses was confirmed by agent-specific qRT-PCR assays. For the first time in this patient cohort, complete genomes of HPgV-1 were acquired and characterized. Phylogenetic analyses indicated that two patients had HPgV-1 genotype 2 and one had HPgV-1 genotype 3. Intra-host genomic variations were described and discussed. Our results highlight the necessity to screen HSCT patients and blood and stem cell donors to reduce the potential risk of HPgV-1 transmission.
