Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6)
Issued Date
2023-09-01
Resource Type
ISSN
17355362
eISSN
17359414
Scopus ID
2-s2.0-85171366326
Journal Title
Research in Pharmaceutical Sciences
Volume
18
Issue
5
Start Page
517
End Page
527
Rights Holder(s)
SCOPUS
Bibliographic Citation
Research in Pharmaceutical Sciences Vol.18 No.5 (2023) , 517-527
Suggested Citation
Ngamratanapaiboon S., Pornchokchai K., Wongpitoonmanachai S., Pholkla P., Srikornvit N., Mo J., Hongthawonsiri P., Yambangyang P., Akrachalanont P. Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6). Research in Pharmaceutical Sciences Vol.18 No.5 (2023) , 517-527. 527. doi:10.4103/1735-5362.383707 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/90199
Title
Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6)
Author's Affiliation
Other Contributor(s)
Abstract
Background and purpose: The use of fluoxetine raises the risk of pancreatic beta-cell dysfunction. However, the specific mechanism behind its mechanism of action in beta cells is unknown. This study investigated the cellular response of MIN6 cells to fluoxetine using untargeted cell-based metabolomics. Experimental approach: Metabolic profiling of MIN6 cells was performed using liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis on samples prepared under optimized conditions, followed by principal component analysis, partial least squares-discriminant analysis, and pair-wise orthogonal projections to latent structures discriminant analyses. Findings/Results: Sixty-six metabolites that had been differentially expressed between the control and fluoxetine-treated groups demonstrated that the citric acid cycle is mainly perturbed by fluoxetine treatment. Conclusion and implications: The current study provides insights into the molecular mechanisms of fluoxetine effects in MIN6 cells.