Fluorescence origami paper–based analytical device based on strand displacement assay for SARS-CoV-2 cDNA detection
2
Issued Date
2025-06-01
Resource Type
ISSN
00263672
eISSN
14365073
Scopus ID
2-s2.0-105006697619
Journal Title
Microchimica Acta
Volume
192
Issue
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microchimica Acta Vol.192 No.6 (2025)
Suggested Citation
Naorungroj S., Mahardika I.H., Huh E., Yukird J., Pasomsub E., Chailapakul O., Shin K. Fluorescence origami paper–based analytical device based on strand displacement assay for SARS-CoV-2 cDNA detection. Microchimica Acta Vol.192 No.6 (2025). doi:10.1007/s00604-025-07228-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/110508
Title
Fluorescence origami paper–based analytical device based on strand displacement assay for SARS-CoV-2 cDNA detection
Corresponding Author(s)
Other Contributor(s)
Abstract
A fluorescence origami paper–based analytical device (Flu-oPAD) based on a strand displacement assay as a point-of-care testing (POCT) sensing platform for DNA detection is presented. This device facilitates multiple steps in a single device, including sample loading, incubation, and washing. The detection zone was immobilized with a 6-FAM-modified probe (F-probe), which formed a complex with a BHQ1-modified probe (Q-probe) to minimize background signals. In the presence of target DNA, hybridization with the F-probe releases the Q-probe, resulting in a fluorescence response proportional to the target DNA concentration. The sensor exhibits good selectivity for target DNA, with a linearity range from 0.1 nM to 10 μM and an experimental detection limit of 0.1 nM, completing all procedures within 15 min. The real-world applicability is demonstrated by successfully detecting complementary DNA (cDNA) from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a cause of the COVID-19 pandemic. This study further introduces a multi-array Flu-oPAD for simultaneous detection of SARS-CoV-2 ORF1ab, N, and E gene cDNAs. This multi-array Flu-oPAD is applied to nasopharyngeal swab samples, showing good agreement with the standard RT-PCR method. Overall, the developed Flu-oPAD has shown great potential as an effective POCT tool for DNA screening. It offers simplicity, portability, accessibility, and cost-effectiveness, offering its potential impact on addressing pressing healthcare needs.