Acclimated chitosan for inorganic nitrogen removal in recirculating aquaculture system for Penaeus vannamei cultivation
1
Issued Date
2025-01-01
Resource Type
ISSN
17551307
eISSN
17551315
Scopus ID
2-s2.0-105008761979
Journal Title
Iop Conference Series Earth and Environmental Science
Volume
1500
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Iop Conference Series Earth and Environmental Science Vol.1500 No.1 (2025)
Suggested Citation
Satanwat P., Boonprasertsakul T., Yocawibun P., Chumtong P., Tapaneeyaworawong P., Powtongsook S., Vanichviriyakit R. Acclimated chitosan for inorganic nitrogen removal in recirculating aquaculture system for Penaeus vannamei cultivation. Iop Conference Series Earth and Environmental Science Vol.1500 No.1 (2025). doi:10.1088/1755-1315/1500/1/012074 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/111012
Title
Acclimated chitosan for inorganic nitrogen removal in recirculating aquaculture system for Penaeus vannamei cultivation
Corresponding Author(s)
Other Contributor(s)
Abstract
This research study aimed to apply acclimated chitosan for inorganic nitrogen removal in the recirculating aquaculture system (RAS). The chitosan flakes were acclimated under conditions with the presence of ammonia and nitrite at a salinity of 15-20 ppt to activate the nitrification activity. Ammonia oxidation was rapidly initiated after one week of acclimation, while nitrite oxidation required ten weeks to complete. The indices of total ammonia nitrogen (TAN) and nitrite removal rates were 0.09±0.03 and 0.05±0.0 mg-N/g-chitosan/day, respectively. The abundance of ammonia-oxidizing bacteria (AOB) amoA gene was relatively constant (8.41 to 15.14 copies/g-chitosan), while nitrite-oxidizing bacteria (NOB) nrxB gene increased to 2.30 × 10<sup>4</sup> copies/g-chitosan. Acclimated chitosan was subsequently installed as a biofilter unit in the RAS for Penaeus vannamei cultivation, operated for two months under a zero-discharge scheme. The control tanks with non-acclimated chitosan showed elevated concentrations of ammonia (0.9 ± 0.3 mg-N/L) and nitrite (4.4 ± 0.8 mg-N/L) during the first month of shrimp cultivation. In contrast, the acclimated chitosan effectively controlled ammonia (0.2 ± 0.1 mg-N/L) and nitrite (0.2 ± 0.2 mg-N/L) concentrations within acceptable limits throughout the cultivation period, resulting in significantly higher shrimp productivity in terms of weight and specific growth rate (SGR).