Study of sigma N2 regulon in Burkholderia Pseudomallei
2
Issued Date
2011
Copyright Date
2011
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xiv, 106 leaves : ill. (some col.)
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Biochemistry))--Mahidol University, 2011
Suggested Citation
Nguyen, Thi Thanh Phuong, 1983- Study of sigma N2 regulon in Burkholderia Pseudomallei . Thesis (M.Sc. (Biochemistry))--Mahidol University, 2011. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/94984
Title
Study of sigma N2 regulon in Burkholderia Pseudomallei
Author(s)
Advisor(s)
Abstract
Burkholderia pseudomallei is the cause of melioidosis, a tropical infection of humans and other animals. The ability of B. pseudomallei to survive under harsh conditions including inside phagocytic cells has been linked to the expression of several pathways. Gram-negative bacteria expresses two families of sigma factors - sigma 70 (RpoS) and sigma 54 (RpoN) - are required for regulating the expression of many genes involved in metabolism and other processes. The RpoN family has been implicated in the expression of nitrogen utilization proteins however the function of the second member of the RpoN family (RpoN2) in B. pseudomallei remains unclear. In this study a role for RpoN2 in controlling the expression of proteins in several pathways was identified. Using complementation and 2D-gel analysis RpoN2 regulation of proteins in amino acid utilization, CysM and HisA involved in cysteine and histidine synthesis respectively were demonstrated. In addition, the level of another 21 proteins was altered in cells lacking in RpoN2, including components of the type III secretion system, bipD and LcrD/SysD. RpoN2 was also found to regulate the expression of katE encoding catalase II, an enzyme produced to protect cells against hydrogen peroxide. Previously, RpoS was identified as the regulator for katE, however our results indicate that the regulation of katE is more complicated and involves RpoN2 as well as RpoS. We propose that RpoS is indirectly regulating katE by controlling the expression of the enhancing binding proteins essential for RpoN function.
Description
Biochemistry (Mahidol University 2011)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Biochemistry
Degree Grantor(s)
Mahidol University