Hyperbaric oxygen therapy improves age induced bone dyshomeostasis in non-obese and obese conditions
1
Issued Date
2022-04-15
Resource Type
ISSN
00243205
eISSN
18790631
Scopus ID
2-s2.0-85124751261
Pubmed ID
35182555
Journal Title
Life Sciences
Volume
295
Rights Holder(s)
SCOPUS
Bibliographic Citation
Life Sciences Vol.295 (2022)
Suggested Citation
Imerb N., Thonusin C., Pratchayasakul W., Arunsak B., Nawara W., Aeimlapa R., Charoenphandhu N., Chattipakorn N., Chattipakorn S.C. Hyperbaric oxygen therapy improves age induced bone dyshomeostasis in non-obese and obese conditions. Life Sciences Vol.295 (2022). doi:10.1016/j.lfs.2022.120406 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83758
Title
Hyperbaric oxygen therapy improves age induced bone dyshomeostasis in non-obese and obese conditions
Other Contributor(s)
Abstract
Aims: To investigate the effects of hyperbaric oxygen therapy (HBOT) on metabolic disturbance, aging and bone remodeling in D-galactose-induced aging rats with and without obesity by determining the metabolic parameters, aging and oxidative stress markers, bone turnover markers, bone microarchitecture, and bone biomechanical strength. Materials and methods: Male Wistar rats were fed either a normal diet (ND; n = 18) or a HFD (n = 12) for 22 weeks. At week 13, vehicle (0.9% NaCl) was injected into ND-fed rats (NDV; n = 6), while 150 mg/kg/day of D-galactose was injected into 12 ND-fed rats (NDD) and 12 HFD-fed rats (HFDD) for 10 weeks. At week 21, rats were treated with either sham (NDVS, NDDS, or HFDDS; n = 6/ group) or HBOT (NDDH, or HFDDH; n = 6/group) for 14 days. Rats were then euthanized. Blood samples, femora, and tibiae were collected. Key findings: Both NDD and HFDD groups developed aging as indicated by increased AGE level, increased inflammation and oxidative stress as shown by raised serum TNF-α and MDA levels, impaired bone remodeling as indicated by an increase in levels of CTX-1, TRACP-5b, and impaired bone structure/strength, when compared with those of the NDVS group. HFD aggravated these indicators of bone dyshomeostasis in D-galactose-treated rats. HBOT restored bone remodeling and bone structure/strength in the NDD group, however HBOT ameliorated bone dyshomeostasis in the HFDD group. Significance: HBOT is a potential intervention to decrease the risk of osteoporosis and bone fracture in aging with or without obesity.