Combining isothermal recombinase polymerase amplification with lateral flow assay for diagnosis of P. cynomolgi malaria infection
4
Issued Date
2023-07-01
Resource Type
eISSN
19352735
Scopus ID
2-s2.0-85165219686
Pubmed ID
37405994
Journal Title
PLoS neglected tropical diseases
Volume
17
Issue
7
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS neglected tropical diseases Vol.17 No.7 (2023) , e0011470
Suggested Citation
Rattaprasert P., Chavananikul C., Fungfuang W., Chavalitshewinkoon-Petmitr P., Limudomporn P. Combining isothermal recombinase polymerase amplification with lateral flow assay for diagnosis of P. cynomolgi malaria infection. PLoS neglected tropical diseases Vol.17 No.7 (2023) , e0011470. doi:10.1371/journal.pntd.0011470 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/88115
Title
Combining isothermal recombinase polymerase amplification with lateral flow assay for diagnosis of P. cynomolgi malaria infection
Author's Affiliation
Other Contributor(s)
Abstract
BACKGROUND: Plasmodium cynomolgi is a nonhuman primate parasite that causes malaria in humans and is transmitted by the Anopheles mosquito. Macaques, the natural hosts of P. cynomolgi, are widely distributed in Asia, especially in Southeast Asia. Anthropogenic land-use changes and wildlife habitat reduction due to local environmental changes, deforestation, urban expansion, and construction increased the frequency of human-macaque-vector interactions and facilitated the emergence of zoonotic malaria, causing an exponential increase in the infection rates in this area. Although microscopic tools are the gold standard for malaria diagnosis, they have very low sensitivity. Therefore, disease control and prevention require rapid, sensitive and accurate diagnostic tests. METHODOLOGY/PRINCIPLE FINDINGS: This study aims to develop a diagnostic method using a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method to specifically diagnose P. cynomolgi. Laboratory validation determined the method's sensitivity and specificity compared to the nested PCR method. The lower limit of detection was 22.14 copies/μl of recombinant plasmid per reaction. The combination method represented 81.82% sensitivity and 94.74% specificity compared to the nested PCR. CONCLUSIONS/SIGNIFICANCE: The diagnostic testing developed in this study combines a recombinase polymerase amplification (RPA) and a lateral flow (LF) strip, offering rapid high sensitivity and specificity. Further development of this technique could make it a promising method for detecting P. cynomolgi.