The effect of Er,Cr:YSGG laser on periodontopathic bacteria elimination: an in vitro study
Issued Date
2022-08-01
Resource Type
ISSN
02688921
eISSN
1435604X
Scopus ID
2-s2.0-85131300671
Pubmed ID
35652988
Journal Title
Lasers in Medical Science
Volume
37
Issue
6
Start Page
2763
End Page
2771
Rights Holder(s)
SCOPUS
Bibliographic Citation
Lasers in Medical Science Vol.37 No.6 (2022) , 2763-2771
Suggested Citation
Sethasathien P., Tantivitayakul P., Teparat-Burana T. The effect of Er,Cr:YSGG laser on periodontopathic bacteria elimination: an in vitro study. Lasers in Medical Science Vol.37 No.6 (2022) , 2763-2771. 2771. doi:10.1007/s10103-022-03573-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87273
Title
The effect of Er,Cr:YSGG laser on periodontopathic bacteria elimination: an in vitro study
Author's Affiliation
Other Contributor(s)
Abstract
In vitro bacterial elimination using the erbium, chromium: yttrium–scandium–gallium–garnet (Er,Cr:YSGG) laser against periodontopathic bacteria was investigated. Bacterial suspensions of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis were spread on agar plates and the Er,Cr:YSGG laser was applied at 40 mJ pulse energy for durations of 30 s, 60 s, and 90 s. The agar plates were incubated, and growth inhibition zones were assessed. Optimal laser irradiation durations to achieve maximal bacterial elimination were evaluated using laser ablation on the bacterial colonies. The remaining viable bacteria were determined by the colony-forming unit (CFU) counting method. Growth inhibition zones were observed at all irradiation durations for both A. actinomycetemcomitans and P. gingivalis. Mean logarithmic values of CFU/ml after bacterial colony irradiation for 0 s (control), 12 s × 1 lap, 24 s × 1 lap, 48 s × 1 lap, and 24 s × 2 laps were 8.82 ± 0.35, 7.31 ± 0.94, 6.32 ± 0.61, 3.17 ± 2.90, and 0.00, respectively, for A. actinomycetemcomitans and 9.83 ± 0.50, 9.42 ± 0.11, 6.90 ± 1.60, 2.33 ± 3.19, and 0.00 for P. gingivalis. Significant differences were found between the control group and the two irradiated groups 48 s × 1 lap and 24 s × 2 laps (p < 0.05), and also between irradiated groups 12 s × 1 lap and 24 s × 2 laps (p < 0.05). An Er,Cr:YSGG laser with power setting 1.5 W and 30 Hz frequency showed potential for bacterial elimination against A. actinomycetemcomitans and P. gingivalis in vitro. Significant bacterial elimination (> 99.99%) was observed after 48 s of irradiation.