Synthesis of M-Ag<inf>3</inf>PO<inf>4</inf>, (M = Se, Ag, Ta) Nanoparticles and Their Antibacterial and Cytotoxicity Study
Issued Date
2022-10-01
Resource Type
ISSN
16616596
eISSN
14220067
Scopus ID
2-s2.0-85139905898
Pubmed ID
36232708
Journal Title
International Journal of Molecular Sciences
Volume
23
Issue
19
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences Vol.23 No.19 (2022)
Suggested Citation
Qureshi F., Nawaz M., Ansari M.A., Khan F.A., Berekaa M.M., Abubshait S.A., Al-Mutairi R., Paul A.K., Nissapatorn V., de Lourdes Pereira M., Wilairatana P. Synthesis of M-Ag<inf>3</inf>PO<inf>4</inf>, (M = Se, Ag, Ta) Nanoparticles and Their Antibacterial and Cytotoxicity Study. International Journal of Molecular Sciences Vol.23 No.19 (2022). doi:10.3390/ijms231911403 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83595
Title
Synthesis of M-Ag<inf>3</inf>PO<inf>4</inf>, (M = Se, Ag, Ta) Nanoparticles and Their Antibacterial and Cytotoxicity Study
Other Contributor(s)
Abstract
Silver Phosphate, Ag3PO4, being a highly capable clinical molecule, an ultrasonic method was employed to synthesize the M-Ag3PO4, (M = Se, Ag, Ta) nanoparticles which were evaluated for antibacterial and cytotoxicity activities post-characterization. Escherichia coli and Staphylococcus aureus were used for antibacterial testing and the effects of sonication on bacterial growth with sub-MIC values of M-Ag3PO4 nanoparticles were examined. The effect of M-Ag3PO4 nanoparticles on human colorectal carcinoma cells (HCT-116) and human cervical carcinoma cells (HeLa cells) was examined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assay and DAPI (4′,6-diamidino-2-phenylindole) staining. Additionally, we analyzed the effect of nanoparticles on normal and non-cancerous human embryonic kidney cells (HEK-293). Ag-Ag3PO4 exhibited enhanced antibacterial activity followed by Ta-Ag3PO4, Ag3PO4, and Se-Ag3PO4 nanoparticles against E. coli. Whereas the order of antibacterial activity against Staphylococcus aureus was Ag3PO4 > Ag-Ag3PO4 > Ta-Ag3PO4 > Se-Ag3PO4, respectively. Percentage inhibition of E. coli was 98.27, 74.38, 100, and 94.2%, while percentage inhibition of S. aureus was 25.53, 80.28, 99.36, and 20.22% after treatment with Ag3PO4, Se-Ag3PO4, Ag-Ag3PO4, and Ta-Ag3PO4, respectively. The MTT assay shows a significant decline in the cell viability after treating with M-Ag3PO4 nanoparticles. The IC50 values for Ag3PO4, Se-Ag3PO4, Ag-Ag3PO4, and Ta-Ag3PO4 on HCT-116 were 39.44, 28.33, 60.24, 58.34 µg/mL; whereas for HeLa cells, they were 65.25, 61.27, 75.52, 72.82 µg/mL, respectively. M-Ag3PO4 nanoparticles did not inhibit HEK-293 cells. Apoptotic assay revealed that the numbers of DAPI stained cells were significantly lower in the M-Ag3PO4-treated cells versus control.