Transcriptome insights into newcastle disease virus-mediated eradication of cholangiocarcinoma cells
Issued Date
2025-05-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-105004397428
Journal Title
PLoS ONE
Volume
20
Issue
5 May
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE Vol.20 No.5 May (2025)
Suggested Citation
Thanyaphoo S., Sae-Lee C., Thaopech W., Amornrit W., Junking M., Yenchitsomanus P.T., Poungvarin N. Transcriptome insights into newcastle disease virus-mediated eradication of cholangiocarcinoma cells. PLoS ONE Vol.20 No.5 May (2025). doi:10.1371/journal.pone.0322307 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/110071
Title
Transcriptome insights into newcastle disease virus-mediated eradication of cholangiocarcinoma cells
Corresponding Author(s)
Other Contributor(s)
Abstract
Newcastle Disease Virus (NDV) has emerged as a promising oncolytic viral therapy for various human cancers; however, its effectiveness against cholangiocarcinoma (CCA) remains unexplored. This study presents the capability of the lentogenic LaSota strain of NDV to eliminate two CCA cell lines, KKU-055 and KKU-100, as well as the potential molecular mechanisms underlying this effect. Comprehensive transcriptome analysis revealed alterations in gene expression within several pathways in CCA cells following exposure to the LaSota strain NDV, including those involved in TNF-alpha signaling via NF-kB, interferon alpha response, apoptosis, and IL-6/JAK/ STAT3 signaling pathways. We remarkably observed a contrasting alteration in the expression of CXCR4, GRAMD1B, IGFBP4, and TGM2 genes in KKU-055 and KKU-100 cells. In addition, gene network analysis highlighted CCNA2, CDK1, DDX58, DHX58, EXO1, GBP1, IFIH1, IFIT1, IFIT2, IFIT3, IRF7, ISIG15, MX1, OAS1, OAS2, PARP9, TOP2A and XAF1 as potential hub genes influencing the response of CCA cells to NDV LaSota strain. Our findings offer evidence supporting the promise of NDV-based therapies as potential strategies for eliminating CCA cells.