Enhancing the Effective Chemotherapy: The Combined Inhibition of Rhinacanthin-C, 5-Fluorouracil, and Etoposide on Oral Cancer Cells
5
Issued Date
2023-07-01
Resource Type
eISSN
2476762X
Scopus ID
2-s2.0-85165934002
Pubmed ID
37505773
Journal Title
Asian Pacific journal of cancer prevention : APJCP
Volume
24
Issue
7
Start Page
2405
End Page
2412
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific journal of cancer prevention : APJCP Vol.24 No.7 (2023) , 2405-2412
Suggested Citation
Klaophimai S., Pouyfung P., Chairatvit K. Enhancing the Effective Chemotherapy: The Combined Inhibition of Rhinacanthin-C, 5-Fluorouracil, and Etoposide on Oral Cancer Cells. Asian Pacific journal of cancer prevention : APJCP Vol.24 No.7 (2023) , 2405-2412. 2412. doi:10.31557/APJCP.2023.24.7.2405 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/88236
Title
Enhancing the Effective Chemotherapy: The Combined Inhibition of Rhinacanthin-C, 5-Fluorouracil, and Etoposide on Oral Cancer Cells
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
OBJECTIVE: To investigate the effects of rhinacanthin-C (Rh-C), 5-FU, and etoposide on growth inhibition, as well as the effects of a combination of these inhibitors on the oral cell lines SCC9 and HSC4. METHODS: Cancer cell growth inhibition and inhibition combination were determined using the SRB assay. Cell viability and early apoptosis were determined using flow cytometry on cells stained with Annexin 5 and PI. Western blotting was performed to study the molecular mechanism of these inhibitors on oral cancer cells. RESULTS: The results showed that etoposide, 5-FU, and Rh-C exhibited more potent anti-proliferative effects on HSC4 cells compared to SCC9 cells in a time- and concentration-dependent manner. The combination of Rh-C and 5-FU was more effective in inhibiting cell growth than the drugs used alone. The combination of 5-FU and Rh-C resulted in a decrease in live HSC4 cells, with the highest percentage of cell death observed at a ratio of 40:6 μM. Furthermore, the combination of 5-FU and Rh-C reduced P-Akt levels leading to a decrease in cell survival. CONCLUSIONS: HSC4 cells were found to be more sensitive to the inhibitory effect of these drugs compared to SCC9 cells. These findings suggest that the use of Rh-C as a complementary therapy with 5-FU may have the potential for the treatment of oral cancer. the underlying mechanisms responsible for this difference in sensitivity between the two cell lines need to be further investigated.