Evolutionary tracks of chromosomal diversification in Trichopsis (Anabantiformes, Osphronemidae) fishes: New insights from a molecular cytogenetic perspective
Issued Date
2023-01-01
Resource Type
ISSN
1412033X
eISSN
20854722
Scopus ID
2-s2.0-85152702607
Journal Title
Biodiversitas
Volume
24
Issue
3
Start Page
1551
End Page
1559
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biodiversitas Vol.24 No.3 (2023) , 1551-1559
Suggested Citation
Mingkwan B., De Menezes Cavalcante Sassi F., Muanglenm N., Pinmongkhonkul S., Pinthong K., Tongnunui S., Yeesin P., Tanomtong A., Liehr T., Cioffi M.D.B., Supiwong W. Evolutionary tracks of chromosomal diversification in Trichopsis (Anabantiformes, Osphronemidae) fishes: New insights from a molecular cytogenetic perspective. Biodiversitas Vol.24 No.3 (2023) , 1551-1559. 1559. doi:10.13057/biodiv/d240327 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81475
Title
Evolutionary tracks of chromosomal diversification in Trichopsis (Anabantiformes, Osphronemidae) fishes: New insights from a molecular cytogenetic perspective
Other Contributor(s)
Abstract
This study investigated the chromosomes of three Trichopsis species (namely Trichopsis pumila, Trichopsis vittata, and Trichopsis schalleri) using conventional (Giemsa stain, Ag-NOR) and molecular cytogenetic techniques. Fluorescence in situ hybridization (FISH) with repetitive DNAs, including 5S and 18S rDNAs, and microsatellites as probes were also performed. Our results indicated a conserved diploid number of 46 (2n) for all analyzed species of both sexes, although varying in their karyotype structure. Furthermore, T. schalleri and T. vittata karyotypes were presented with only acrocentric chromosomes (46a), while T. pumila had a submetacentric pair in addition to acrocentric ones (2sm+44a). Positive Ag-NOR sites were differentially found in each species adjacent to the acrocentric chromosome's centromeric and/or interstitial sub-centromeric regions. Both 5S and 18S rDNAs vary between the analyzed species, but all were localized in multiple sites. However, the distribution pattern of microsatellites differed in each species, mostly scattering at peri-centromeric and telomeric regions. These findings show that Trichopsis species, while having a conserved diploid number, differ significantly in the distribution of repetitive sequences in their karyotypes, particularly for rDNAs. These species-specific patterns can be useful for characterizing and identifying further different species and examining the evolutionary mechanisms that drove the evolution of these fishes' karyotypes.