A Simple and Applicable Method for Human Platelet Lysate Preparation Using Citrate Blood
Issued Date
2022-09-01
Resource Type
ISSN
00075027
eISSN
19437730
Scopus ID
2-s2.0-85137137130
Pubmed ID
35016203
Journal Title
Lab Medicine
Volume
53
Issue
5
Start Page
E109
End Page
E112
Rights Holder(s)
SCOPUS
Bibliographic Citation
Lab Medicine Vol.53 No.5 (2022) , E109-E112
Suggested Citation
Khongjaroensakun N., Paisooksantivatana K., Santiwatana S., Tawonsawatruk T., Kusolthammarat K., Kadegasem P., Tangbubpha N., Chantaraamporn J., Chuansumrit A. A Simple and Applicable Method for Human Platelet Lysate Preparation Using Citrate Blood. Lab Medicine Vol.53 No.5 (2022) , E109-E112. E112. doi:10.1093/labmed/lmab116 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/85572
Title
A Simple and Applicable Method for Human Platelet Lysate Preparation Using Citrate Blood
Other Contributor(s)
Abstract
Objectives: To determine and compare the platelet growth factors in human platelet lysate (HPL) prepared from citrated whole blood, with final centrifugations at 4oC and 25oC. Methods: We collected specimens of citrated whole blood from 27 healthy volunteers. The platelet-rich plasma (PRP) was separated to prepare the HPL, which was further divided into 2 portions for the final centrifugation, at 4oC and 25oC, respectively. Platelet growth factors were measured and compared between the 2 groups. Results: All platelet growth factors were higher than those in PRP prepared from citrated whole blood. Moreover, the final centrifugation at 25oC resulted in noninferiority of platelet-growth-factor level. Conclusion: This study provided a simple method for small-volume of HPL preparation using only 10-15 mL of citrated whole blood. Further, the entire process of centrifugation can be performed at room temperature of 25oC, which is more applicable than lower temperatures for other laboratories.