Electrochemical lateral-flow device for rapid COVID-19 antigen-diagnostic testing
Issued Date
2023-08-01
Resource Type
ISSN
15675394
eISSN
1878562X
Scopus ID
2-s2.0-85152102589
Pubmed ID
37054603
Journal Title
Bioelectrochemistry
Volume
152
Rights Holder(s)
SCOPUS
Bibliographic Citation
Bioelectrochemistry Vol.152 (2023)
Suggested Citation
Deenin W., Yakoh A., Pimpitak U., Pasomsub E., Rengpipat S., Crespo G.A., Chaiyo S. Electrochemical lateral-flow device for rapid COVID-19 antigen-diagnostic testing. Bioelectrochemistry Vol.152 (2023). doi:10.1016/j.bioelechem.2023.108438 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81372
Title
Electrochemical lateral-flow device for rapid COVID-19 antigen-diagnostic testing
Other Contributor(s)
Abstract
Antigen test kits (ATK) are extensively utilized for screening and diagnosing COVID-19 because they are easy to operate. However, ATKs exhibit poor sensitivity and cannot detect low concentrations of SARS-CoV-2. Herein, we present a new, highly sensitive, and selective device obtained by combining the principle of ATKs with electrochemical detection for COVID-19 diagnosis, which can be quantitatively assessed using a smartphone. An electrochemical test strip (E-test strip) was constructed by attaching a screen-printed electrode inside a lateral-flow device to exploit the remarkable binding affinity of SARS-CoV-2 antigen to ACE2. The ferrocene carboxylic acid attached to SARS-CoV-2 antibody acts as an electroactive species when it binds to SARS-CoV-2 antigen in the sample before it flows continuously to the ACE2-immobilization region on the electrode. Electrochemical-assay signal intensity on smartphones increased proportionally to the concentration of SARS-CoV-2 antigen (LOD = 2.98 pg/mL, under 12 min). Additionally, the application of the single-step E-test strip for COVID-19 screening was demonstrated using nasopharyngeal samples, and the results were consistent with those obtained using the gold standard (RT-PCR). Therefore, the sensor demonstrated excellent performance in assessing and screening COVID-19, and it can be used professionally to accurately verify diagnostic data while remaining rapid, simple, and inexpensive.