IDENTIFICATION OF SARS-COV-2 LINEAGES USING A COST-EFFECTIVE MASSARRAY® SYSTEM
Issued Date
2022-09-09
Resource Type
ISSN
01251562
eISSN
26975718
Scopus ID
2-s2.0-85140826477
Journal Title
Southeast Asian Journal of Tropical Medicine and Public Health
Volume
53
Issue
5
Start Page
513
End Page
529
Rights Holder(s)
SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health Vol.53 No.5 (2022) , 513-529
Suggested Citation
Panthan B. IDENTIFICATION OF SARS-COV-2 LINEAGES USING A COST-EFFECTIVE MASSARRAY® SYSTEM. Southeast Asian Journal of Tropical Medicine and Public Health Vol.53 No.5 (2022) , 513-529. 529. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87245
Title
IDENTIFICATION OF SARS-COV-2 LINEAGES USING A COST-EFFECTIVE MASSARRAY® SYSTEM
Author(s)
Other Contributor(s)
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19. Identification of virus lineages is key to tracking the chains of transmission and for monitoring variants of interest as well as variants of concern. This study aimed to develop and validate a cost-effective method to determine SARS-CoV-2 lineages of virus samples from the first wave of the pandemic in Thailand (January-May 2020). A lineage-specific single nucleotide polymorphism (SNP) genotyping panel of SARS-CoV-2 was designed based on genomic surveillance data generated in the first wave. Viral RNA specimens obtained from the Department of Pathology, Faculty of Medicine Ramathibodi Hospital were analyzed by a MassARRAY® platform in comparison to a next generation sequencing of the virus genome. The MassARRAY® system was able to identify the SARS-CoV-2 lineages with high accuracy supporting its application as a rapid and cost-effective method for identification of circulating COVID-19 variants in the country, and has the ability to accommodate new SARS-CoV-2 variants by simple modifications of the assay protocol.