Production and bioactivities of nanoparticulated and ultrasonic-degraded levan generated by Erwinia tasmaniensis levansucrase in human osteosarcoma cells
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Issued Date
2022-11-30
Resource Type
ISSN
01418130
eISSN
18790003
Scopus ID
2-s2.0-85137886621
Pubmed ID
36115448
Journal Title
International Journal of Biological Macromolecules
Volume
221
Start Page
1121
End Page
1129
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Biological Macromolecules Vol.221 (2022) , 1121-1129
Suggested Citation
Charoenwongpaiboon T., Wangpaiboon K., Septham P., Jiamvoraphong N., Issaragrisil S., Pichyangkura R., Lorthongpanich C. Production and bioactivities of nanoparticulated and ultrasonic-degraded levan generated by Erwinia tasmaniensis levansucrase in human osteosarcoma cells. International Journal of Biological Macromolecules Vol.221 (2022) , 1121-1129. 1129. doi:10.1016/j.ijbiomac.2022.09.096 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83554
Title
Production and bioactivities of nanoparticulated and ultrasonic-degraded levan generated by Erwinia tasmaniensis levansucrase in human osteosarcoma cells
Author's Affiliation
Other Contributor(s)
Abstract
Levan is a bioactive polysaccharide that can be synthesized by various microorganisms. In this study, the physicochemical properties and bioactivity of levan synthesized by recombinant levansucrase from Erwinia tasmaniensis were investigated. The synthesis conditions, including the enzyme concentration, substrate concentration, and temperature, were optimized. The obtained levan generally appeared as a cloudy suspension. However, it could transform into a hydrogel at concentrations exceeding 10 % (w/v). Then, ultrasonication was utilized to reduce the molecular weight and increase the bioavailability of levan. Dynamic light scattering (DLS) and gel permeation chromatography (GPC) indicated that the size of levan was significantly decreased by ultrasonication, whereas Fourier transform infrared spectroscopy, 1H-nuclear magnetic resonance, and X-ray powder diffraction revealed that the chemical structure of levan was not changed. Finally, the bioactivities of both levan forms were examined using human osteosarcoma (Saos-2) cells. The result clearly illustrated that sonicated levan had higher antiproliferative activity in Saos-2 cells than original levan. Sonicated levan also activated Toll-like receptor expression at the mRNA level. These findings suggested the important beneficial applications of sonicated levan for the development of cancer therapies.