Molecular and morphometric differentiation of secondary filariasis vector Coquillettidia mosquitoes (Diptera: Culicidae) in Thailand
Issued Date
2023-08-01
Resource Type
ISSN
15671348
eISSN
15677257
Scopus ID
2-s2.0-85163452091
Pubmed ID
37257802
Journal Title
Infection, Genetics and Evolution
Volume
112
Rights Holder(s)
SCOPUS
Bibliographic Citation
Infection, Genetics and Evolution Vol.112 (2023)
Suggested Citation
Laojun S., Changbunjong T., Sumruayphol S., Chaiphongpachara T. Molecular and morphometric differentiation of secondary filariasis vector Coquillettidia mosquitoes (Diptera: Culicidae) in Thailand. Infection, Genetics and Evolution Vol.112 (2023). doi:10.1016/j.meegid.2023.105452 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87837
Title
Molecular and morphometric differentiation of secondary filariasis vector Coquillettidia mosquitoes (Diptera: Culicidae) in Thailand
Other Contributor(s)
Abstract
Coquillettidia mosquitoes are important nuisance-biting pests and a vector of brugian filariasis in Thailand. However, comprehensive information about these mosquitoes remains unavailable such as molecular and morphometric differences among species. The lack of vector knowledge on Coquillettidia species could affect future disease control. This study aims to investigate differences in molecular variations based on mitochondrial cytochrome oxidase subunit I (COI) gene and wing geometric traits of three Coquillettidia species, namely Cq. crassipes, Cq. nigrosignata, and Cq. ochracea in Thailand. The results of molecular analyses revealed the differences among three Coquillettidia species. The genetic difference measure based on the Kimura two-parameter model among three Coquillettidia species showed low intraspecific distances (0%–3.05%) and large interspecific distances (10.10%–12.41%). The values of intra- and inter-genetic differences of three Coquillettidia species did not overlap which showed the existence of a barcoding gap indicating the efficiency of the identification based on the COI gene. As with molecular analysis, the landmark-based geometric morphometrics approach based on wing shape analysis indicated three distinct species groups which were supported by the high total performance score of cross-validated classification (97.16%). These results provide the first evidence of taxonomic signal based on molecular and wing geometric differences to support species identification and biological variations of Coquillettidia mosquitoes in Thailand for understanding these rare vector mosquitoes in depth and leading to effective further mosquito control.