Influence of Lithium- and Zinc-Containing Bioactive Glasses on Pulpal Regeneration
Issued Date
2023-01-01
Resource Type
ISSN
13057456
eISSN
13057464
Scopus ID
2-s2.0-85162780863
Journal Title
European Journal of Dentistry
Rights Holder(s)
SCOPUS
Bibliographic Citation
European Journal of Dentistry (2023)
Suggested Citation
Tran A.T.L., Sukajintanakarn C., Senawongse P., Sritanaudomchai H., Ruangsawasdi N., Lapthanasupkul P., Kitkumthorn N., Monmaturapoj N., Khamsut C., Naruphontjirakul P., Pongprueksa P. Influence of Lithium- and Zinc-Containing Bioactive Glasses on Pulpal Regeneration. European Journal of Dentistry (2023). doi:10.1055/s-0042-1758789 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87768
Title
Influence of Lithium- and Zinc-Containing Bioactive Glasses on Pulpal Regeneration
Other Contributor(s)
Abstract
Objective To evaluate the potential of modified bioactive glasses containing lithium and zinc as pulp capping materials by investigating the odontogenic differentiation and mineralization response in the tooth culture model. Materials and Methods Lithium- and zinc-containing bioactive glasses (45S5.1Li, 45S5.5Li, 45S5.1Zn, 45S5.5Zn, 45S5.1Zn sol-gel, and 45S5.5Zn sol-gel), fibrinogen-thrombin, and biodentine were prepared to assess Axin2 gene expression at 0, 30 minutes, 1 hour, 12 hours, and 1 day and DSPP gene expression at 0, 3, 7, and 14 days in stem cells from human exfoliated deciduous teeth (SHEDs) using qRT-PCR. The experimental bioactive glasses incorporated with fibrinogen-thrombin and biodentine were placed on the pulpal tissue in the tooth culture model. Histology and immunohistochemistry were analyzed at 2 weeks and 4 weeks. Results Axin2 gene expression for all experimental groups was significantly higher than the control at 12 hours. The DSPP gene expression for all experimental groups was significantly higher than the control at 14 days. The presence of mineralization foci was significantly higher at 4 weeks for the modified bioactive glasses 45S5.5Zn, 45S5.1Zn sol-gel, and 45S5.5Zn sol-gel as well as Biodentine compared with the fibrinogen-thrombin control. Conclusion Lithium - and zinc - containing bioactive glasses increased Axin2 and DSPP gene expression in SHEDs and can potentially enhance pulp mineralization and regeneration. Zinc - containing bioactive glasses are a promising candidate to be used as pulp capping materials.