Effects of Ovarian Parasite Marteilioides chungmuensis on the Reproduction of the Pacific Oyster Crassostrea gigas Assessed by Histology and Enzyme-Linked Immunosorbent Assay (ELISA)
3
Issued Date
2022-01-01
Resource Type
ISSN
1598141X
Scopus ID
2-s2.0-85144688406
Journal Title
Ocean and Polar Research
Volume
44
Issue
4
Start Page
319
End Page
329
Rights Holder(s)
SCOPUS
Bibliographic Citation
Ocean and Polar Research Vol.44 No.4 (2022) , 319-329
Suggested Citation
Limpanont Y., Kang H.S., Cho Y.G., Shin J.S., Kajino N., Kim J.H., Hong H.K., Choi K.S. Effects of Ovarian Parasite Marteilioides chungmuensis on the Reproduction of the Pacific Oyster Crassostrea gigas Assessed by Histology and Enzyme-Linked Immunosorbent Assay (ELISA). Ocean and Polar Research Vol.44 No.4 (2022) , 319-329. 329. doi:10.4217/OPR.2022023 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83353
Title
Effects of Ovarian Parasite Marteilioides chungmuensis on the Reproduction of the Pacific Oyster Crassostrea gigas Assessed by Histology and Enzyme-Linked Immunosorbent Assay (ELISA)
Other Contributor(s)
Abstract
The paramyxean parasite Marteilioides chungmuensis infects the cytoplasm of the eggs of Pacific oysters Crassostrea gigas, resulting in spawning failure of the infected females. Such infected eggs appear as bump-like nodules on the body in late fall when most of the uninfected females complete spawning. In this study, we estimated the quantity of the infected eggs using an enzyme-linked immunosorbent assay (ELISA), which is destroyed by M. chungmuensis parasitism. In December, the infected oysters collected from Tong-young on the south coast exhibited numerous yellowish bump-like nodules as signs of infection. In histology, the infected oysters exhibited mature eggs in the follicle, which were heavily infiltrated by hemocytes. ELISA indicated that the infected egg mass accounted for 7.52±5.50 percent of the body weight, suggesting the ovarian parasite causes substantial reproductive loss. Histology also indicated that the infected oysters are in a poor nutritional condition, as the digestive gland atrophy (DGA) level is comparatively higher than the uninfected oyster. The total carbohydrate contents in the infected oysters (108.68±44.41 mg/g dry wt) were significantly lower than in uninfected oysters (269.76±50.97 mg/g dry wt), suggesting that M. chungmuensis parasitism also affected the energy storage capacity of the host during the resting stage.