Glucose absorption activity and gene expression of sugar transporters in the trophotaenia of the viviparous teleost Xenotoca eiseni
Issued Date
2023-11-01
Resource Type
ISSN
03044165
eISSN
18728006
Scopus ID
2-s2.0-85171468010
Journal Title
Biochimica et Biophysica Acta - General Subjects
Volume
1867
Issue
11
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biochimica et Biophysica Acta - General Subjects Vol.1867 No.11 (2023)
Suggested Citation
Iida A., Tsuda N., Yoshida J., Nomura J., Ratanayotha A., Kawai T., Hondo E. Glucose absorption activity and gene expression of sugar transporters in the trophotaenia of the viviparous teleost Xenotoca eiseni. Biochimica et Biophysica Acta - General Subjects Vol.1867 No.11 (2023). doi:10.1016/j.bbagen.2023.130464 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/90200
Title
Glucose absorption activity and gene expression of sugar transporters in the trophotaenia of the viviparous teleost Xenotoca eiseni
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
In viviparous reproductive systems, nutrient transfer from mother to embryo plays a critical role in the generation of offspring. Herein, we investigated the mother-to-embryo nutrient transfer machinery in the viviparous teleost Xenotoca eiseni, which belongs to the family Goodeidae. The intraovarian embryo absorbs maternal supplements via the hindgut-derived placental structure termed the trophotaenia. Tracer analysis indicated that the trophotaenia can take up glucose analogs in ex vivo cultured embryos. The candidate genes for absorption, sglt1, glut2, atp1a, and atp1b, were determined from published transcriptomes. These genes were expressed in the trophotaenia of X. eiseni embryos. Fluorescent immunohistochemistry of Na+/K+ ATPase indicated the polarity of epithelial cells in the trophotaenia. The presented evidence suggests that the epithelial cell layer transports monosaccharides from the apical membrane of epithelial cells in a basolateral direction. Taken together, this study provides insight into how maternal fish maintain their offspring during gestation and will aid in the development of strategies to improve offspring generation in these fish.