Sulfated Galactans from Gracilaria fisheri with Supplementation of Octanoyl Promote Wound Healing Activity In Vitro and In Vivo
Issued Date
2022-12-01
Resource Type
ISSN
16165187
eISSN
16165195
Scopus ID
2-s2.0-85137945304
Pubmed ID
36066490
Journal Title
Macromolecular Bioscience
Volume
22
Issue
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
Macromolecular Bioscience Vol.22 No.12 (2022)
Suggested Citation
Rudtanatip T., Somintara S., Sakaew W., El-Abid J., Cano M.E., Jongsomchai K., Wongprasert K., Kovensky J. Sulfated Galactans from Gracilaria fisheri with Supplementation of Octanoyl Promote Wound Healing Activity In Vitro and In Vivo. Macromolecular Bioscience Vol.22 No.12 (2022). doi:10.1002/mabi.202200172 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/85071
Title
Sulfated Galactans from Gracilaria fisheri with Supplementation of Octanoyl Promote Wound Healing Activity In Vitro and In Vivo
Other Contributor(s)
Abstract
Sulfated galactans (SG) isolated from Gracilaria fisheri is partially degraded (DSG), and subsequentially supplemented with octanoyl (DSGO) and sulfate (DSGS) groups. The molecular weights of DSG, DSGO, and DSGS are 7.87, 152.79, and 97.07 kDa, respectively. The modification is confirmed using FTIR and NMR, while in vitro wound healing activity is assessed using scratched wound fibroblasts. The results reveal that DSGO exhibits highest percentage of wound closure in scratched fibroblast L929 cells. Furthermore, DSGO is able to promote proliferation and accelerate migration of scratched fibroblasts, which correspond to the regulation of proteins and mRNA (Ki67, p-FAK, vimentin, and E-cadherin) determined by Western blotting and qPCR analysis. The superior wound healing activity of DSGO is also confirmed in excision wound of rats. The results demonstrate that DSGO significantly enhances the percentage of wound closure, re-epithelialization, and collagen arrangement, increases α-smoth muscle actin (α-SMA) and vimentin expression, and decreases that of tumor necrosis factor-α (TNF-α) at the wound site. The results suggest that degraded SG supplemented with medium-chain fatty acids of octanoyl group may pass through the membrane, subsequently activating the mediators associated with proliferation and migration of fibroblasts, which can potentially lead to the promotion of wound healing activity.