Genome mining reveals novel biosynthetic gene clusters in entomopathogenic bacteria
Issued Date
2023-11-25
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85177768745
Pubmed ID
38007490
Journal Title
Scientific reports
Volume
13
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific reports Vol.13 No.1 (2023) , 20764
Suggested Citation
Meesil W., Muangpat P., Sitthisak S., Rattanarojpong T., Chantratita N., Machado R.A.R., Shi Y.M., Bode H.B., Vitta A., Thanwisai A. Genome mining reveals novel biosynthetic gene clusters in entomopathogenic bacteria. Scientific reports Vol.13 No.1 (2023) , 20764. doi:10.1038/s41598-023-47121-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/91318
Title
Genome mining reveals novel biosynthetic gene clusters in entomopathogenic bacteria
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
Senckenberg Gesellschaft für Naturforschung
Shenzhen Institute of Advanced Technology
Naresuan University
Philipps-Universität Marburg
King Mongkut's University of Technology Thonburi
Max Planck Institute for Terrestrial Microbiology
Goethe-Universität Frankfurt am Main
Université de Neuchâtel
Zentrum für Synthetische Mikrobiologie Synmikro
Senckenberg Gesellschaft für Naturforschung
Shenzhen Institute of Advanced Technology
Naresuan University
Philipps-Universität Marburg
King Mongkut's University of Technology Thonburi
Max Planck Institute for Terrestrial Microbiology
Goethe-Universität Frankfurt am Main
Université de Neuchâtel
Zentrum für Synthetische Mikrobiologie Synmikro
Other Contributor(s)
Abstract
The discovery of novel bioactive compounds produced by microorganisms holds significant potential for the development of therapeutics and agrochemicals. In this study, we conducted genome mining to explore the biosynthetic potential of entomopathogenic bacteria belonging to the genera Xenorhabdus and Photorhabdus. By utilizing next-generation sequencing and bioinformatics tools, we identified novel biosynthetic gene clusters (BGCs) in the genomes of the bacteria, specifically plu00736 and plu00747. These clusters were identified as unidentified non-ribosomal peptide synthetase (NRPS) and unidentified type I polyketide synthase (T1PKS) clusters. These BGCs exhibited unique genetic architecture and encoded several putative enzymes and regulatory elements, suggesting its involvement in the synthesis of bioactive secondary metabolites. Furthermore, comparative genome analysis revealed that these BGCs were distinct from previously characterized gene clusters, indicating the potential for the production of novel compounds. Our findings highlighted the importance of genome mining as a powerful approach for the discovery of biosynthetic gene clusters and the identification of novel bioactive compounds. Further investigations involving expression studies and functional characterization of the identified BGCs will provide valuable insights into the biosynthesis and potential applications of these bioactive compounds.