Role of LATS kinase on the mesenchymal stem cells differentiation to chondrocytes
Issued Date
2024
Copyright Date
2020
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
viii, 38 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Immunology))--Mahidol University, 2020
Suggested Citation
Wannawat Khotchawan Role of LATS kinase on the mesenchymal stem cells differentiation to chondrocytes. Thesis (M.Sc. (Immunology))--Mahidol University, 2020. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/92130
Title
Role of LATS kinase on the mesenchymal stem cells differentiation to chondrocytes
Author(s)
Advisor(s)
Abstract
The Hippo signalling pathway is important for controlling the organ size and tissue homeostasis. The non-canonical Hippo pathway was shown to control the osteogenesis and adipogenesis of Mesenchymal stem cells (MSCs). However, their role in chondrogenesis is poorly disclosed. Therefore, this present study aims to investigate the role of LATS kinase, one of the Hippo pathway core-kinases, on MSC differentiation to chondrocytes. Lysophosphatidic acid (LPA) was used to modulate the expression of LATS in placenta and chorion-derived MSCs at different time points during chondrogenic induction. The differentiation result was assessed by the expression of chondrogenic specific genes and proteins. The treatment of MSCs with LPA result in the reduced LATS1 and LATS2 protein expressions in the dose-dependent manners. The cell from all treatments can be differentiated to chondrocytes. However, the significant down-regulation of MSCs specific marker and the up-regulation of chondrocyte-related gene were found in the LPA treated cells when compared to the non-treated control. Interestingly, chondrocyte specific collagen Type II (COL2A1) was significantly increased in the LPA-treated cells. This result suggests that the inhibition of LATS kinase enhances chondrogenic differentiation of MSCs. The result of this finding could be beneficial to the patients with cartilage degeneration.
Description
Immunology (Mahidol University 2020)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Medicine Siriraj Hospital
Degree Discipline
Immunology
Degree Grantor(s)
Mahidol University