Utilization of tissue culture technique for propagation of Melia spp.
Issued Date
2024
Copyright Date
1992
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
vi, 46 leaves : ill. (some col.)
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1992
Suggested Citation
Suriya Jansaengsri Utilization of tissue culture technique for propagation of Melia spp.. Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1992. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/100735
Title
Utilization of tissue culture technique for propagation of Melia spp.
Alternative Title(s)
การใช้เทคนิคการเพาะเลี้ยงเนื้อเยื่อการขยายพันธุ์ไม้เลื่ยน
Author(s)
Advisor(s)
Abstract
Tissue culture technique applied for mass propagation of Melia spp. is possible. The modified LS basal medium used in this study is composed of macronutrients, micronutrients from LS medium, vitamins of B5 proposed by Gamborg, 6 g/l agar and 10% coconut water. The explants used in this experiment were young leaf, petiole, internode, terminal bud and axillary bud from young plant. The optimun concentration of BA for multiple shoot induction from axillary bud and terminal bud was 1 mg/l. Callus cultures can be readily initiated and proliferated from petiole and internode in the basal medium supplemented with 1 mg/l NAA plus 1 mg/l BA but no callus mass was observed from young leaf explant. The derived callus cultured on basal medium supplemented with 1 mg/l BA regenerated shoots which had a vigorous growth in basal medium with 0.25 regale BA. The individual shootlets regenerated vigorous roots within 7 - 10 days after immersion of the basal cut end into the solution of 1 mg/l IAA and 1 mg/l IBA for 1 day, then transferral to basal medium 85% of the treated shoots rooted. One month old plantlets were then transplanted into pots filled with sterilized vermiculite under high humidity conditions. The survival percentage was about 21%, most of the mortality being due to fungus infection.
Description
Environmental Biology (Mahidol University 1992)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Environmental Biology
Degree Grantor(s)
Mahidol University