A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy
3
Issued Date
2022-08-15
Resource Type
ISSN
00032670
eISSN
18734324
Scopus ID
2-s2.0-85133706132
Pubmed ID
35934369
Journal Title
Analytica Chimica Acta
Volume
1221
Rights Holder(s)
SCOPUS
Bibliographic Citation
Analytica Chimica Acta Vol.1221 (2022)
Suggested Citation
Saisuk W., Srisawat C., Yoksan S., Dharakul T. A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy. Analytica Chimica Acta Vol.1221 (2022). doi:10.1016/j.aca.2022.340134 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83632
Title
A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy
Author(s)
Other Contributor(s)
Abstract
In the present study, a molecular beacon biosensor was developed to enable efficient detection of the viral RNAs using a previously described HyCaSD platform. The HyCaSD molecular beacon probes were labeled with the Cy5 and BHQ3 at each end of the hairpin probes. The fluorescent signal was detected immediately only when the molecular beacon probes specifically hybridized to the target sequence and unfolded their hairpin structures. This combination greatly improved the sensitivity with LOD of 100 copy equivalents per reaction (around 20-fold greater than the original HyCaSD). In addition, our MB-based HyCaSD demonstrated a single-step, single-tube and actual-time RNA detection procedure, thereby bringing it a major step closer to point-of-care diagnostic applications for viral infectious diseases.