A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite
Issued Date
2023-08-01
Resource Type
eISSN
20794991
Scopus ID
2-s2.0-85168859004
Journal Title
Nanomaterials
Volume
13
Issue
16
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nanomaterials Vol.13 No.16 (2023)
Suggested Citation
Madaci A., Suwannin P., Raffin G., Hangouet M., Martin M., Ferkous H., Bouzid A., Bausells J., Elaissari A., Errachid A., Jaffrezic-Renault N. A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite. Nanomaterials Vol.13 No.16 (2023). doi:10.3390/nano13162316 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/89280
Title
A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite
Other Contributor(s)
Abstract
In this paper, a microconductometric sensor has been designed, based on a chitosan composite including alcohol dehydrogenase—and its cofactor—and gold nanoparticles, and was calibrated by differential measurements in the headspace of aqueous solutions of ethanol. The role of gold nanoparticles (GNPs) was crucial in improving the analytical performance of the ethanol sensor in terms of response time, sensitivity, selectivity, and reproducibility. The response time was reduced to 10 s, compared to 21 s without GNPs. The sensitivity was 416 µS/cm (v/v%)−1 which is 11.3 times higher than without GNPs. The selectivity factor versus methanol was 8.3, three times higher than without GNPs. The relative standard deviation (RSD) obtained with the same sensor was 2%, whereas it was found to be 12% without GNPs. When the air from the operator’s mouth was analyzed just after rinsing with an antiseptic mouthwash, the ethanol content was very high (3.5 v/v%). The background level was reached only after rinsing with water.