Bioassay-Guided Isolation and Identification of Antiplasmodial Compounds from the Stem Bark of Clausena excavata
Issued Date
2023-10-01
Resource Type
eISSN
14390221
Scopus ID
2-s2.0-85172034419
Pubmed ID
37414059
Journal Title
Planta medica
Volume
89
Issue
12
Start Page
1165
End Page
1169
Rights Holder(s)
SCOPUS
Bibliographic Citation
Planta medica Vol.89 No.12 (2023) , 1165-1169
Suggested Citation
Seephonkai P., Kaewtong C., Wangchuk P., Jearawuttanakul K., Kanjanasirirat P., Borwornpinyo S., Khulmanee T., Seephonkai P., Patrapuvich R. Bioassay-Guided Isolation and Identification of Antiplasmodial Compounds from the Stem Bark of Clausena excavata. Planta medica Vol.89 No.12 (2023) , 1165-1169. 1169. doi:10.1055/a-2112-6631 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/90268
Title
Bioassay-Guided Isolation and Identification of Antiplasmodial Compounds from the Stem Bark of Clausena excavata
Other Contributor(s)
Abstract
Clausena excavata is a medicinal plant widely distributed in Southeast Asia. It is used for a variety of indications, including to treat malaria. In our present study, a phytochemical study of the methanol extract from the stem bark of C. excavata led to the isolation of five pyranocoumarins, nordentatin (1: ), dentatin (2: ), kinocoumarin (3: ), clausarin (4: ), and clausenidin (5: ), and a coumarin, 8-hydroxy-3″,4″-dihydrocapnolactone-2',3'-diol (6: ). The isolation of compound 6: from C. excavata and the antiplasmodial activities against a multidrug-resistant K1 strain of Plasmodium falciparum of 1, 3: , and 5: were reported for the first time. Compounds 3: and 4: exhibited potent antiplasmodial activities with EC50 values of 1.10 and 0.58 µM, respectively, while 1: and 5: had EC50 values of 5.62 and 7.15 µM, respectively. A prenyl group attached to the C-3 or C-12 position on the pyranocoumarin ring probably plays an important role on the activity. A hydroxyl group at the C-10 position is also likely to enhance the activity.