Seed storage of Ipomoea alba L. in DOA genebank Thailand

Abstract

A study was conducted on the influence of seed moisture and storage temperature for seed germination and seed storage period of Ipomoea alba L. This study was separated into four experiments based on the condition of storage temperature as follows; room temperature, 5, -10, and -196°C. These experiments were designed in split plot design with 4 replicates, including 2 factors as main plot with 4 levels of percent moisture in seed at 6, 8, 10, and 15.4% and sub plot with 7 levels of period for seed storage at 0, 3, 6, 9, 12, 15, and 18 months. The results showed that seed moisture and period of seed storage affected the percent of seed germination of I. alba in all storage temperatures. The room temperature with 6, 8, and 10% of seed moisture (storage for 18 months) showed the seed germination at 77, 82, and 60%, respectively; while, non-reduced seed moisture could be stored about 9 months. The seed moisture did not affect seed germination of I. alba which was kept at low temperature for 18 months. In addition, seed moisture of 6, 8, 10 and 15.4% at 5, -10, and -196°C exhibited seed germination as (85, 83, 85, and 49%), (94, 83, 89, and 78%), and (83, 75, 75, and 67%), respectively.