Genetic diversity and host specificity of Blastocystis in reptiles, Eastern Thailand
Issued Date
2024-07-01
Resource Type
ISSN
03044017
eISSN
18732550
Scopus ID
2-s2.0-85194852289
Journal Title
Veterinary Parasitology
Volume
329
Rights Holder(s)
SCOPUS
Bibliographic Citation
Veterinary Parasitology Vol.329 (2024)
Suggested Citation
Sutthikornchai C., Abu A., Pintong A.r., Chiabchalard R., Nokkaew W., Toompong J., Popruk S., Stensvold C.R. Genetic diversity and host specificity of Blastocystis in reptiles, Eastern Thailand. Veterinary Parasitology Vol.329 (2024). doi:10.1016/j.vetpar.2024.110218 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/98670
Title
Genetic diversity and host specificity of Blastocystis in reptiles, Eastern Thailand
Corresponding Author(s)
Other Contributor(s)
Abstract
Blastocystis inhabits the digestive tracts of a diverse range of hosts. Transmission patterns, including host specificity, and the clinical and public health significance of Blastocystis in humans remain poorly understood. This study aimed to investigate the distribution and genetic diversity of Blastocystis in herbivorous and carnivorous reptiles in Eastern Thailand. A total of 501 faecal samples were collected from 363 iguanas, 79 bearded dragons, 50 tortoises, and nine snakes in an animal breeding farm in Chonburi Province, Eastern Thailand. Detection and differentiation of Blastocystis was based on amplification, sequencing, and phylogenetic analysis of specific small subunit (SSU) ribosomal RNA genes from faecal DNA extracted from the samples. Altogether 101/501 samples (20 %) were polymerase chain reaction (PCR) and sequencing-positive for Blastocystis, 90 (89 %) of which were from iguanas; the remaining positive samples were from African spurred tortoise (n=6), Bearded dragon (n=3), Leopard tortoise (n=1), and Red-footed tortoise (n=1). Phylogenetic analysis revealed that most of the Blastocystis sequences from iguanas were largely similar, and they were distinct from those of the tortoises. Subtype 17 was found in the three bearded dragons and likely reflected Blastocystis from prey animals. This is the largest survey of Blastocystis in reptiles to date. Remarkable differences in Blastocystis colonization rates and genetic diversity were observed between iguanas and other reptile orders, and what was considered Blastocystis colonization was only observed in herbivorous reptiles.