Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand
Issued Date
2024-01-01
Resource Type
eISSN
20544200
Scopus ID
2-s2.0-85192584122
Pubmed ID
38716477
Journal Title
Global health, epidemiology and genomics
Volume
2024
Rights Holder(s)
SCOPUS
Bibliographic Citation
Global health, epidemiology and genomics Vol.2024 (2024) , 8872463
Suggested Citation
Cheewapat R., Redkimned J., Lekuthai S., Kitti T., Tasanapak K., Thanwisai A., Sitthisak S., Sornda T., Impheng H., Onsurathum S., Leungtongkam U., Lamlertthon S., Kucharoenphaibul S., Wongwigkarn J., Singkum P., Chanchaithong P., Thummeepak R. Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand. Global health, epidemiology and genomics Vol.2024 (2024) , 8872463. doi:10.1155/2024/8872463 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98365
Title
Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand
Corresponding Author(s)
Other Contributor(s)
Abstract
This study utilized integrative bioinformatics' tools together with phenotypic assays to understand the whole-genome features of a carbapenem-resistant international clone II Acinetobacter baumannii AB073. Overall, we found the isolate to be resistant to seven antibiotic classes, penicillins, β-lactam/β-lactamase inhibitor combinations, cephalosporins, carbapenems, aminoglycosides, fluoroquinolones, and folate pathway antagonists. These resistance phenotypes are related to various chromosomal-located antibiotic resistance determinants involved in different mechanisms such as reduced permeability, antibiotic target protection, antibiotic target alteration, antibiotic inactivation, and antibiotic efflux. IC2 A. baumannii AB073 could not transfer antibiotic resistance by conjugation experiments. Likewise, mobilome analysis found that AB073 did not carry genetic determinants involving horizontal gene transfer. Moreover, this isolate also carried multiple genes associated with the ability of iron uptake, biofilm formation, immune invasion, virulence regulations, and serum resistance. In addition, the genomic epidemiological study showed that AB073-like strains were successful pathogens widespread in various geographic locations and clinical sources. In conclusion, the comprehensive analysis demonstrated that AB073 contained multiple genomic determinants which were important characteristics to classify this isolate as a successful international clone II obtained from Thailand.