Ferulic acid enhances insulin secretion by potentiating L-type Ca<sup>2+</sup> channel activation
Issued Date
2023-01-01
Resource Type
ISSN
20954964
Scopus ID
2-s2.0-85143963656
Pubmed ID
36481247
Journal Title
Journal of Integrative Medicine
Volume
21
Issue
1
Start Page
99
End Page
105
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Integrative Medicine Vol.21 No.1 (2023) , 99-105
Suggested Citation
Ruamyod K., Watanapa W.B., Kakhai C., Nambundit P., Treewaree S., Wongsanupa P. Ferulic acid enhances insulin secretion by potentiating L-type Ca<sup>2+</sup> channel activation. Journal of Integrative Medicine Vol.21 No.1 (2023) , 99-105. 105. doi:10.1016/j.joim.2022.11.003 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82625
Title
Ferulic acid enhances insulin secretion by potentiating L-type Ca<sup>2+</sup> channel activation
Author's Affiliation
Other Contributor(s)
Abstract
Objective: To investigate the effect of ferulic acid, a natural compound, on pancreatic beta cell viability, Ca2+ channels, and insulin secretion. Methods: We studied the effects of ferulic acid on rat insulinoma cell line viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide viability assay. The whole-cell patch-clamp technique and enzyme-linked immunosorbent assay were also used to examine the action of ferulic acid on Ca2+ channels and insulin secretion, respectively. Results: Ferulic acid did not affect cell viability during exposures up to 72 h. The electrophysiological study demonstrated that ferulic acid rapidly and concentration-dependently increased L-type Ca2+ channel current, shifting its activation curve in the hyperpolarizing direction with a decreased slope factor, while the voltage dependence of inactivation was not affected. On the other hand, ferulic acid have no effect on T-type Ca2+ channels. Furthermore, ferulic acid significantly increased insulin secretion, an effect inhibited by nifedipine and Ca2+-free extracellular fluid, confirming that ferulic acid-induced insulin secretion in these cells was mediated by augmenting Ca2+ influx through L-type Ca2+ channel. Our data also suggest that this may be a direct, nongenomic action. Conclusion: This is the first electrophysiological demonstration that acute ferulic acid treatment could increase L-type Ca2+ channel current in pancreatic β cells by enhancing its voltage dependence of activation, leading to insulin secretion.