Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera
Issued Date
2022-10-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-85140143980
Pubmed ID
36227939
Journal Title
PLoS ONE
Volume
17
Issue
10 October
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE Vol.17 No.10 October (2022)
Suggested Citation
Uthailak N., Adisakwattana P., Thiangtrongjit T., Limpanont Y., Chusongsang P., Chusongsang Y., Tanasarnprasert K., Reamtong O. Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera. PLoS ONE Vol.17 No.10 October (2022). doi:10.1371/journal.pone.0275992 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87647
Title
Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera
Author's Affiliation
Other Contributor(s)
Abstract
Schistosomiasis is a neglected tropical disease caused by an infection of the parasitic flatworms schistosomes. Schistosoma mekongi is a restricted Schistosoma species found near the Mekong River, mainly in southern Laos and northern Cambodia. Because there is no vaccine or effective early diagnosis available for S. mekongi, additional biomarkers are required. In this study, serum biomarkers associated with S. mekongi-infected mice were identified at 14-, 28-, 42-, and 56-days post-infection. Circulating proteins and antigens of S. mekongi in mouse sera were analyzed using mass spectrometry-based proteomics. Serine protease inhibitors and macrophage erythroblast attacher were down-regulated in mouse sera at all infection timepoints. In addition, 54 circulating proteins and 55 antigens of S. mekongi were identified. Notable circulating proteins included kyphoscoliosis peptidase and putative tuberin, and antigens were detected at all four infection timepoints, particularly in the early stages (12 days). The putative tuberin sequence of S. mekongi was highly similar to homologs found in other members of the genus Schistosoma and less similar to human and murine sequences. Our study provided the identity of promising diagnostic biomarkers that could be applicable in early schistosomiasis diagnosis and vaccine development.