Host-finding behavior of Strongyloides stercoralis infective larvae to sodium cation, human serum and sweat
Issued Date
2004
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Masataka Koga, Supaporn Nuamtanong, สุภาพร น่วมทะนง, Paron Dekumyoy, พารณ ดีคำย้อย, Tippayarat Yoonuan, ทิพยรัตน์ อยู่นวล, Wanna Maipanich, วรรณา ไมพานิช, Wichit Rojekittikhun, วิชิต โรจน์กิตติคุณ, Jitra Waikagul, จิตรา ไวคกุล (2004). Host-finding behavior of Strongyloides stercoralis infective larvae to sodium cation, human serum and sweat. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63402
Title
Host-finding behavior of Strongyloides stercoralis infective larvae to sodium cation, human serum and sweat
Other Contributor(s)
Abstract
The host-finding behavior of Strongyloides stercoralis infective larvae was examined by in vitro agarose assay method. As human body fluid contains 0.85% (ca 0.15 molar) NaCl, various concentrations of sodium chloride, from 0.5M to 0.01M (7 steps), were examined. Many larvae were attracted at concentrations between 0.5 and 0.05M of sodium chloride. The concentration of 0.05M attracted the most larvae. The concentration of 0.02M of sodium chloride showed greatly reduced larval attraction compared with 0.05M. Therefore, the threshold concentration was determined as 0.05M. Then, 0.05M of chemicals were examined in a further experiment. Chloride compounds (NaCl, KCl, CaCl2, MgCl2) were investigated. These chemicals are components of human body fluids. Distilled water was used as the control in all experiments. Only sodium chloride attracted the larvae. Next, alkaline compounds were examined [NaOH, KOH, Ca(OH)2, and Mg(OH)2]. Larvae accumulated only at the NaOH site. The results suggested that the Na cation is important for larval attraction. A high pH value did not influence attraction at all. Next, human serum was tested. The human serum used was from normal serum to 1:32 diluted sera by distilled water (7 steps). Hierarchical attraction was seen according to serum concentration. Next, human sweat was collected from a limited zone of chest skin where only eccrine glands were distributed. Non-diluted sweat attracted the most larvae. Sweat might act as one of the most probable factors for infection by this skin-penetrating nematode.
Description
Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.175.