Evaluating lignin degradation under limited oxygen conditions by bacterial isolates from forest soil
Issued Date
2024-06-10
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85195623249
Pubmed ID
38858437
Journal Title
Scientific reports
Volume
14
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific reports Vol.14 No.1 (2024) , 13350
Suggested Citation
Sumranwanich T., Amosu E., Chankhamhaengdecha S., Phetruen T., Loktumraks W., Ounjai P., Harnvoravongchai P. Evaluating lignin degradation under limited oxygen conditions by bacterial isolates from forest soil. Scientific reports Vol.14 No.1 (2024) , 13350. doi:10.1038/s41598-024-64237-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98824
Title
Evaluating lignin degradation under limited oxygen conditions by bacterial isolates from forest soil
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Corresponding Author(s)
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Abstract
Lignin, a heterogeneous aromatic polymer present in plant biomass, is intertwined with cellulose and hemicellulose fibrils, posing challenges to its effective utilization due to its phenolic nature and recalcitrance to degradation. In this study, three lignin utilizing bacteria, Klebsiella sp. LEA1, Pseudomonas sp. LEA2, and Burkholderia sp. LEA3, were isolated from deciduous forest soil samples in Nan province, Thailand. These isolates were capable of growing on alkali lignin and various lignin-associated monomers at 40 °C under microaerobic conditions. The presence of Cu2+ significantly enhanced guaiacol oxidation in Klebsiella sp. LEA1 and Pseudomonas sp. LEA2. Lignin-related monomers and intermediates such as 2,6-dimethoxyphenol, 4-vinyl guaiacol, 4-hydroxybenzoic acid, benzoic acid, catechol, and succinic acid were detected mostly during the late stage of incubation of Klebsiella sp. LEA1 and Pseudomonas sp. LEA2 in lignin minimal salt media via GC-MS analysis. The intermediates identified from Klebsiella sp. LEA1 degradation suggested that conversion and utilization occurred through the β-ketoadipate (ortho-cleavage) pathway under limited oxygen conditions. The ability of these bacteria to thrive on alkaline lignin and produce various lignin-related intermediates under limited oxygen conditions suggests their potential utility in oxygen-limited processes and the production of renewable chemicals from plant biomass.