Molecular Epidemiology of Penicillinase-Producing Neisseria gonorrhoeae Isolates and Their bla<inf>TEM-135</inf> Gene Variant in Bangkok, Thailand, 2015–2017
Issued Date
2023-01-01
Resource Type
ISSN
13446304
eISSN
18842836
Scopus ID
2-s2.0-85151044697
Pubmed ID
36450575
Journal Title
Japanese Journal of Infectious Diseases
Volume
76
Issue
2
Start Page
126
End Page
134
Rights Holder(s)
SCOPUS
Bibliographic Citation
Japanese Journal of Infectious Diseases Vol.76 No.2 (2023) , 126-134
Suggested Citation
Nokchan N., Nitayanon P., Tribuddharat C. Molecular Epidemiology of Penicillinase-Producing Neisseria gonorrhoeae Isolates and Their bla<inf>TEM-135</inf> Gene Variant in Bangkok, Thailand, 2015–2017. Japanese Journal of Infectious Diseases Vol.76 No.2 (2023) , 126-134. 134. doi:10.7883/yoken.JJID.2022.484 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/82535
Title
Molecular Epidemiology of Penicillinase-Producing Neisseria gonorrhoeae Isolates and Their bla<inf>TEM-135</inf> Gene Variant in Bangkok, Thailand, 2015–2017
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Penicillinase-producing Neisseria gonorrhoeae (PPNG) possessing blaTEM-135 is a serious public health threat. With only a single change in the amino acid sequence, blaTEM-135 could evolve into a TEM-type extended-spectrum beta-lactamase (ESBL), which hydrolyzes extended-spectrum cephalosporins, including ceftriaxone and cefixime. We investigated the molecular epidemiological characteristics, types of plasmids in PPNG isolates, and prevalence of PPNG clinical isolates producing TEM-135 beta-lactamases. N. gonorrhoeae multi-antigen sequence typing (NG-MAST) was used to determine the molecular epidemiological characteristics of 99 PPNG isolates collected from 2015 to 2017. A mismatch amplification mutation assay was used to examine the blaTEM-135 gene prevalence. Of the 89 identified NG-MAST sequence types, 65 (73.0%) were novel. Only 17.7% (43/243) of PPNG isolates belonged to 16 genogroups. The most frequent plasmid was African, followed by Rio/Toronto, and Asian. The blaTEM-135 allele was found in Rio/Toronto plasmids. The blaTEM-135 allele was present in 23.2% (23/99) of the PPNG isolates. PPNG isolates expressing TEM-135 beta-lactamase exhibited significantly higher penicillin MIC (minimum inhibitory concentration) values than TEM-1 PPNG isolates. The PPNG isolates showed high genetic diversity and a high proportion of blaTEM-135 alleles. Mutation of the blaTEM-135 allele is worrisome as only one mutation could cause TEM-1 to evolve into an ESBL variant that degrades ceftriaxone. Ongoing surveillance of blaTEM-135 and new PPNG isolates is imperative.