A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
Issued Date
2022-05-01
Resource Type
ISSN
1940087X
DOI
Scopus ID
2-s2.0-85130720385
Pubmed ID
35635460
Journal Title
Journal of Visualized Experiments
Volume
2022
Issue
183
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Visualized Experiments Vol.2022 No.183 (2022)
Suggested Citation
Sa-Ngiamsuntorn K., Thongsri P., Pewkliang Y., Borwornpinyo S., Wongkajornsilp A. A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target. Journal of Visualized Experiments Vol.2022 No.183 (2022). doi:10.3791/63761 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83736
Title
A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
Other Contributor(s)
Abstract
Hepatitis B virus (HBV) infection has been considered a crucial risk factor for hepatocellular carcinoma. Current treatment can only lessen the viral load but not result in complete remission. An efficient hepatocyte model for HBV infection would offer a true-to-life viral life cycle that would be crucial for the screening of therapeutic agents. Most available anti-HBV agents target lifecycle stages post viral entry but not before viral entry. This protocol details the generation of a competent hepatocyte model capable of screening for therapeutic agents targeting pre-viral entry and post viral entry lifecycle stages. This includes the targeting of sodium taurocholate cotransporting polypeptide (NTCP) binding, cccDNA formation, transcription, and viral assembly based on imHC or HepaRG as host cells. Here, the HBV entry inhibition assay used curcumin to inhibit HBV binding and transporting functions via NTCP. The inhibitors were evaluated for binding affinity (KD) with NTCP using isothermal titration calorimetry (ITC)-a universal tool for HBV drug screening based on thermodynamic parameters.