Effect of diuresis on ammonium excretion after unilateral ureteral obstruction in rats
1
Issued Date
2024
Copyright Date
1992
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
x, 126 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Physiology))--Mahidol University, 1992
Suggested Citation
Sopapan Chaekuntode Effect of diuresis on ammonium excretion after unilateral ureteral obstruction in rats. Thesis (M.Sc. (Physiology))--Mahidol University, 1992. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/99336
Title
Effect of diuresis on ammonium excretion after unilateral ureteral obstruction in rats
Alternative Title(s)
อิทธิพลของการเพิ่มอัตราการไหลของปัสสาวะต่อการขับแอมโมเนียในหนูภายหลังการอุดตันของท่อปัสสาวะเพียงข้างเดียว
Author(s)
Abstract
The present studies indirectly investigated the site of tubular defect that leads to a reduction or ammonium excretion by the damaged kidney after 24 hours of unilateral ureteral obstruction (UUO) in rat. After the released of UUO, the kidney was unable to lower urine pH. The urinary pH of post-obstructed kidney (POK) was 7.28 as compared to 6.11 in the contralateral control kidney (CCK). The fractional bicarbonate excretion of the POR was 10 times the value of the CCK. The rate of ammonium excretion (U(,NH4) and ammonium index (U(,NH4 V/GFR) were both markedly reduced. Thus,the impaired urine acidification was due to reduction in hydrogen ion secretion, bicarbonate reabsorption and ammonium excretion. Induction of diuresis by loop diuretic, furosemide, resulted in increasing ammonium index in both kidneys. However, the Henles loop of the POK may be defective since the degree of the increasing was smaller than those observed in the CCK. Normal saline infusion also lead to increasing ammonium indices in both CCK and POK. However, this increased ammonium index the POK after saline loading was only 37% of the CCK in the normal condition and it was less than the corresponding value after furosemide infusion. Thus, there may be a reduction in ammonium generation and secretion at proximal tubule after UUO. After right nephrectomy (RNx), both methods of diuresis induction resulted in a marked increase in GFR and also ammonium indices. It was suggested that the ammonium index of the POK is in part depending on GFR, which may vary with the number of functioning nephron. Thus, the reduction of ammonium excretion after 24 hrs UUO be due to the reduction in ammonium generation and secretion at proximal tubule. Furthermore, ammonium index is increased irrespect of the methods of diuresis induction which strengthen the conclusion that the defective site of ammonium excretion after 24 hrs UUO may be mainly at proximal tubule and the reabsorption at loop of Henle may not be the major contributing factor in this condition.
Description
Physiology (Mahidol University 1992)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Physiology
Degree Grantor(s)
Mahidol University