3D-printed microgels supplemented with dentin matrix molecules as a novel biomaterial for direct pulp capping
Issued Date
2023-03-01
Resource Type
ISSN
14326981
eISSN
14363771
Scopus ID
2-s2.0-85140654264
Pubmed ID
36287273
Journal Title
Clinical Oral Investigations
Volume
27
Issue
3
Start Page
1215
End Page
1225
Rights Holder(s)
SCOPUS
Bibliographic Citation
Clinical Oral Investigations Vol.27 No.3 (2023) , 1215-1225
Suggested Citation
Cunha D., Souza N., Moreira M., Rodrigues N., Silva P., Franca C., Horsophonphong S., Sercia A., Subbiah R., Tahayeri A., Ferracane J., Yelick P., Saboia V., Bertassoni L. 3D-printed microgels supplemented with dentin matrix molecules as a novel biomaterial for direct pulp capping. Clinical Oral Investigations Vol.27 No.3 (2023) , 1215-1225. 1225. doi:10.1007/s00784-022-04735-z Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81814
Title
3D-printed microgels supplemented with dentin matrix molecules as a novel biomaterial for direct pulp capping
Other Contributor(s)
Abstract
Objectives: To develop a 3D-printed, microparticulate hydrogel supplemented with dentin matrix molecules (DMM) as a novel regenerative strategy for dental pulp capping. Materials and methods: Gelatin methacryloyl microgels (7% w/v) mixed with varying concentrations of DMM were printed using a digital light projection 3D printer and lyophilized for 2 days. The release profile of the DMM-loaded microgels was measured using a bicinchoninic acid assay. Next, dental pulp exposure defects were created in maxillary first molars of Wistar rats. The exposures were randomly capped with (1) inert material − negative control, (2) microgels, (3) microgels + DMM 500 µg/ml, (4) microgels + DMM 1000 µg/ml, (5) microgels + platelet-derived growth factor (PDGF 10 ng/ml), or (6) MTA (n = 15/group). After 4 weeks, animals were euthanized, and treated molars were harvested and then processed to evaluate hard tissue deposition, pulp tissue organization, and blood vessel density. Results: All the specimens from groups treated with microgel + 500 µg/ml, microgel + 1000 µg/ml, microgel + PDGF, and MTA showed the formation of organized pulp tissue, tertiary dentin, newly formed tubular and atubular dentin, and new blood vessel formation. Dentin bridge formation was greater and pulp necrosis was less in the microgel + DMM groups compared to MTA. Conclusions: The 3D-printed photocurable microgels doped with DMM exhibited favorable cellular and inflammatory pulp responses, and significantly more tertiary dentin deposition. Clinical relevance: 3D-printed microgel with DMM is a promising biomaterial for dentin and dental pulp regeneration in pulp capping procedures.