Antimalarial target vulnerability of the putative Plasmodium falciparum methionine synthase
Issued Date
2024-01-01
Resource Type
eISSN
21678359
Scopus ID
2-s2.0-85184747825
Journal Title
PeerJ
Volume
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
PeerJ Vol.12 (2024)
Suggested Citation
Leela N., Prommana P., Kamchonwongpaisan S., Taechalertpaisarn T., Shaw P.J. Antimalarial target vulnerability of the putative Plasmodium falciparum methionine synthase. PeerJ Vol.12 (2024). doi:10.7717/peerj.16595 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/97240
Title
Antimalarial target vulnerability of the putative Plasmodium falciparum methionine synthase
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Abstract
Background. Plasmodium falciparum possesses a cobalamin-dependent methionine synthase (MS). MS is putatively encoded by the PF3D7_1233700 gene, which is orthologous and syntenic in Plasmodium. However, its vulnerability as an antimalarial target has not been assessed. Methods. We edited the PF3D7_1233700 and PF3D7_0417200 (dihydrofolate reductase-thymidylate synthase, DHFR-TS) genes and obtained transgenic P. falciparum parasites expressing epitope-tagged target proteins under the control of the glmS ribozyme. Conditional loss-of-function mutants were obtained by treating transgenic parasites with glucosamine. Results. DHFR-TS, but not MS mutants showed a significant proliferation defect over 96 h, suggesting that P. falciparum MS is not a vulnerable antimalarial target.